Receptor-like cytoplasmic kinases (RLCKs) represent a large family of proteins in plants. In Arabidopsis and rice, several RLCKs in subfamily VII (RLCKs-VII) have been implicated in pathogen-associated molecular pattern-triggered immunity and basal resistance against bacterial and fungal pathogens. However, little is known about roles of RLCKs-VII of the important crop common wheat (Triticum aestivum) in immune responses. Here, we isolated a RLCK-VII-encoding gene from wheat, designated as TaRLCK1B, and investigated its role in host immune response to infection of a necrotrophic fungus Rhizoctonia cerealis that is a major pathogen of sharp eyespot, a destructive disease of wheat. RNA-sequencing and RT-qPCR analyses showed that transcriptional level of TaRLCK1B was significantly higher in sharp eyespot-resistant wheat cultivars than in susceptible wheat cultivars. The gene transcription was rapidly and markedly elevated in the resistant wheat cultivars by R. cerealis infection. The TaRLCK1B protein was closely related to OsRLCK176, a rice resistance-related RLCKs-VII, with 84.03% identity. Virus-induced gene silencing plus wheat response to R. cerealis assay results indicated that silencing of TaRLCK1 impaired resistance to R. cerealis. Meantime, silencing of TaRLCK1 significantly elevated both the content of H₂O₂ (a major kind of reactive oxygen species, ROS) and the transcriptional level of the ROS-generating enzyme-encoding gene RBOH, but repressed the expression of the ROS-scavenging enzyme-encoding gene CAT1 at 18 hours after inoculation (hai) with R. cerealis. Taken together, these data suggested that TaRLCK1B was required for the early immune response of wheat to R. cerealis through modulating ROS signaling in wheat.

受体样细胞质激酶（RLCK）代表植物中的一大类蛋白质。在拟南芥和水稻中，VII亚家族的几个RLCK（RLCKs-VII）与病原体相关的分子模式触发的免疫力和对细菌和真菌病原体的基础抗性有关。然而，关于重要农作物普通小麦（Triticum aestivum）的RLCKs-VII在免疫应答中的作用知之甚少。在这里，我们从小麦中分离出一个编码RLCK-VII的基因，命名为TaRLCK1B，并研究了其在宿主对坏死性真菌Rhizoctonia grainis感染的免疫反应中的作用。那是尖锐的眼点的主要病原体，这是小麦的毁灭性疾病。RNA测序和RT-qPCR分析显示，在耐点眼尖的小麦品种中，TaRLCK1B的转录水平明显高于易感小麦品种。在抗性小麦品种中，通过谷物麦芽孢杆菌感染，基因转录迅速并显着升高。TaRLCK1B蛋白与水稻抗性相关的RLCKs-VII OsRLCK176紧密相关，同一性为84.03％。病毒诱导的基因沉默加小麦响应R.禾谷测定结果表明的那沉默TaRLCK1受损电阻R.禾谷。同时，TaRLCK1沉默显着提高了H ₂ O ₂的含量（一种主要的活性氧种类ROS）和ROS生成酶编码基因RBOH的转录水平，但抑制了ROS清除酶编码基因CAT1的表达接种谷物谷胱甘肽（hai）后18小时。总之，这些数据表明，TaRLCK1B被要求对小麦的早期免疫应答R.禾谷通过调节ROS在小麦的信令。