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Exogenous supplementation of melatonin alters representative organic acids and enzymes of respiratory cycle as well as sugar metabolism during arsenic stress in two contrasting indica rice cultivars
Journal of Biotechnology ( IF 4.1 ) Pub Date : 2020-10-14 , DOI: 10.1016/j.jbiotec.2020.10.013
Santanu Samanta 1 , Ankur Singh 1 , Aditya Banerjee 1 , Aryadeep Roychoudhury 1
Affiliation  

The objective of the present investigation was to understand the impact of exogenously applied melatonin on mitochondrial respiration and sugar metabolism in two contrasting rice cultivars, viz., Khitish (arsenic-susceptible) and Muktashri (arsenic-tolerant) under arsenic-stress. Melatonin effectively restored the level of organic acids like pyruvic acid, malic acid and more particularly citric acid by 33 % in Khitish which were lowered during arsenic-stress, whereas their levels were further elevated in Muktashri to provide energy for defence against arsenic-induced injury. Arsenic-exposure led to a significant inhibition in enzyme activities as well as corresponding transcript level of key respiratory enzymes, viz., pyruvate dehydrogenase, citrate synthase, isocitrate dehydrogenase, succinate dehydrogenase and malate dehydrogenase, intriguingly more prominently in case of Khitish. Conversely, melatonin supplementation, irrespective of cultivars, considerably improved the activity of the above enzymes and corresponding gene expressions during stress, indicating acceleration in the rate of Krebs cycle. Melatonin supplementation also stimulated the accumulation of total soluble sugars by 62 % and 25 %, reducing sugars by 50 % and 44 % and non-reducing sugars by 75 % and 14 % in Khitish and Muktashri respectively, concomitant with higher activities of acid invertase, sucrose synthase and sucrose phosphate synthase enzymes, along with the expression of corresponding genes. Enhanced starch accumulation via regulation of alpha amylase and starch phosphorylase activities and gene expression, by melatonin also contributed towards better stress tolerance. Overall, this work illustrated the efficacy of melatonin in the regulation of representative organic acids and enzymes of respiratory cycle along with starch and sugar metabolism in rice cultivars under arsenic toxicity.



中文翻译:

外源性补充褪黑激素改变两个对比籼稻品种在砷胁迫期间呼吸循环的代表性有机酸和酶以及糖代谢

本调查的目的是了解外源性应用褪黑激素对两种对比水稻品种的线粒体呼吸和糖代谢的影响,即在砷胁迫下的 Khitish(砷敏感)和 Muktashri(耐砷)。褪黑激素有效地将 Khitish 中有机酸(如丙酮酸、苹果酸,尤其是柠檬酸)的水平恢复了 33%,这些水平在砷胁迫期间降低,而 Muktashri 中的水平进一步升高,为防御砷引起的损伤提供能量. 砷暴露导致酶活性的显着抑制以及关键呼吸酶的相应转录水平,即丙酮酸脱氢酶、柠檬酸合酶、异柠檬酸脱氢酶、琥珀酸脱氢酶和苹果酸脱氢酶,有趣的是,在 Khitish 的情况下更加突出。相反,无论品种如何,褪黑激素的补充显着提高了上述酶的活性和胁迫期间相应基因的表达,表明三羧酸循环速率加快。在 Khitish 和 Muktashri 中,补充褪黑激素还分别刺激了 62% 和 25% 的总可溶性糖的积累,减少了 50% 和 44% 的还原糖以及 75% 和 14% 的非还原糖,伴随着酸性转化酶的更高活性,蔗糖合酶和蔗糖磷酸合酶,以及相应基因的表达。褪黑激素通过调节 α 淀粉酶和淀粉磷酸化酶活性和基因表达来增强淀粉积累,这也有助于提高抗逆性。总体,

更新日期:2020-11-13
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