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Mild-cold water swimming does not exacerbate white adipose tissue browning and brown adipose tissue activation in mice
Journal of Physiology and Biochemistry ( IF 3.4 ) Pub Date : 2020-10-14 , DOI: 10.1007/s13105-020-00771-z
Jhonattan Toniatto da Silva 1 , Paola Sanches Cella 1 , Mayra Tardelli de Jesus Testa 1 , Luiz Augusto Perandini 1, 2 , William T Festuccia 2 , Rafael Deminice 1 , Patricia Chimin 1
Affiliation  

The present study investigated the effects of swimming physical training either thermoneutral or below thermoneutral water temperature on white (WAT) and brown (BAT) adipose tissue metabolism, morphology, and function. C57BL/6J male mice (n = 40; weight 25.3 ± 0.1 g) were divided into control (CT30), cold control (CT20), trained (TR30), and cold trained (TR20) groups. Swimming training consisted of 30-min exercise at 30°C (control) or 20°C (cold) water temperature. After 8-week training, adipose tissues were excised and inguinal (ingWAT) and BAT were processed for histology, lipolysis, and protein contents of total OXPHOS, PGC1α, and UCP1 by western blotting analysis. Swimming training reduced body weight gain independently of water temperature (P < 0.05). ingWAT mass was decreased for TR30 in comparison to other groups (P < 0.05), while for BAT, there was a significant increase in CT20 in relation to CT30, and both trained groups were significantly increased in relation to control groups (P < 0.05). ingWAT mean adipocyte area was smaller for trained groups, and seemed to present multilocular adipocytes. Lipolytic activity and protein content of UCP1, PGC1α, and mitochondrial markers were increased in trained groups for ingWAT (P < 0.05), independent of water temperature (P > 0.05), and these patterns were not observed for BAT (P > 0.05). Our findings suggest that mild-cold water exposure and swimming physical exercise seem to, independently, promote browning in ingWAT with no effects on BAT; however, the association of exercise and mild-cold water did not exacerbate these effects.



中文翻译:

温和的水游泳不会加剧小鼠白色脂肪组织的褐变和棕色脂肪组织的活化

本研究调查了游泳运动训练对中性(WAT)和棕色(BAT)脂肪组织代谢,形态和功能的影响,无论是热中性水温还是低于热中性水温。将C57BL / 6J雄性小鼠(n  = 40;体重25.3±0.1 g)分为对照组(CT30),冷对照组(CT20),训练组(TR30)和冷训练组(TR20)。游泳训练包括在30°C(对照)或20°C(冷)的水温下进行30分钟的运动。经过8周的训练后,切除了脂肪组织,并通过蛋白质印迹分析对腹股沟(ingWAT)和BAT进行了组织学,脂解以及总OXPHOS,PGC1α和UCP1的蛋白质含量的处理。游泳训练可减少体重增加,与水温无关(P <0.05)。与其他组相比,TR30的ingWAT质量降低了(P  <0.05),而BAT的CT20的CT20相对于CT30的显着增加,并且两个受训的组与对照组相比均明显增加(P  <0.05) 。ingWAT对于训练有素的人群,平均脂肪细胞面积较小,并且似乎呈现多眼脂肪细胞。训练组ingWAT的脂多糖活性和UCP1,PGC1α和线粒体标志物的蛋白质含量增加(P  <0.05),与水温无关(P  > 0.05),而BAT则没有观察到这些模式(P > 0.05)。我们的发现表明,温和的冷水暴露和游泳运动似乎可以独立地促进ingWAT中的褐变,而不会影响BAT。然而,运动与中度冷水的结合并没有加剧这些影响。

更新日期:2020-10-14
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