Dental implants are the most innovative and superior treatment modality for tooth replacement. However, titanium implants still suffer from insufficient antibacterial capability and peri-implant diseases remain one of the most common and intractable complications. To prevent peri-implant diseases, a composite coating containing a new antibacterial agent, (Z-)-4-bromo-5-(bromomethylene)-2(5H)-furanone (BBF) was fabricated on titanium. This study was designed to investigate the antibacterial activity of the composite coating against two common peri-implant pathogens (Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans). The morphology of the composite coating showed that BBF-loaded poly(L-lactic acid) nanospheres were well-distributed in the pores of the microarc oxidation coating, and cross-linked with each other and the wall pores by gelatin. A release study indicated that the antibacterial coating could sustain the release of BBF for 60 d, with a slight initial burst release occurring during the first 4 h. The antibacterial rate of the composite coating for adhering bacteria was the highest (over 97%) after 1 d and over 90% throughout a 30-day incubation period. The total fluorescence intensity of the composite coating was the lowest, and the vast majority of the fluorescence was red (dead bacteria). Moreover, real-time polymerase chain reaction analysis confirmed that the relative gene expression of the adherent bacteria on the composite coating was down-regulated. It was therefore concluded that the composite coating fabricated on titanium, which showed excellent and relatively long-term antibacterial activity against Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, is a potential and promising strategy to be applied on dental implants for the prevention of peri-implant diseases.

种植牙是牙齿置换中最具创新性和优越性的治疗方式。然而，钛种植体仍然存在抗菌能力不足的问题，种植体周围疾病仍然是最常见和最难治的并发症之一。为了预防种植体周围疾病，在钛上制备了一种含有新型抗菌剂 (Z-)-4-bromo-5-(bromomethylene)-2(5H)-furanone (BBF) 的复合涂层。本研究旨在研究复合涂层对两种常见的种植体周围病原体（牙龈卟啉单胞菌和放线放线杆菌）的抗菌活性。）。复合涂层的形貌表明负载BBF的聚(L-乳酸)纳米球在微弧氧化涂层的孔隙中分布良好，并通过明胶相互交联和壁孔。一项释放研究表明，抗菌涂层可以维持 BBF 的释放 60 d，在前 4 小时内发生轻微的初始爆发释放。复合涂层对附着细菌的抑菌率在1 d后最高（超过97%），在30 d的潜伏期内均超过90%。复合涂层的总荧光强度最低，绝大多数荧光为红色（死菌）。而且，实时聚合酶链反应分析证实复合涂层上附着细菌的相对基因表达下调。因此得出结论，在钛上制备的复合涂层显示出优异且相对长期的抗菌活性Porphyromonas gingivalis和Actinobacillus actinomycetemcomitans是一种潜在且有前景的策略，可应用于牙种植体预防种植体周围疾病。