A survey of nematodes associated with terrestrial slugs was conducted in residential gardens, nurseries, greenhouses and agricultural sites located in and around Edmonton, Alberta, Canada. A total of 2406 slugs were collected from 82 sites. Slugs were decapitated and cadavers were incubated for two weeks, with emerging nematodes removed and processed for identification. Nematodes were identified using molecular sequence data for the 18S ribosomal DNA. Nematodes were recovered from 20 of the 82 sites surveyed, with 24.4% of the slugs infected with nematodes. A total of seven nematodes were identified to species level, includingCaenorhabditis elegans,Panagrolaimus papillosus,Pellioditis typica,Pelodera pseudoteres,Rhabditella axei,Rhabditoides inermiformisandPhasmarhabditis californica. An additional four specimens were identified to genus level, includingOscheiussp. (9),Pristionchussp.,Rhabditissp. andRhabditophanessp. (1). The two most common nematode species wereC. elegansandP. pseudoteres. The facultative parasite,P. californica, was recovered from a singleArion rufusspecimen, collected from a seasonal nursery. To our knowledge, this study represents the first survey of slug-associated nematodes in Canada.

中文翻译：

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加拿大艾伯塔省埃德蒙顿地区与陆生蛞蝓有关的线虫

在加拿大艾伯塔省埃德蒙顿及其周边地区的住宅花园、苗圃、温室和农业场所对与陆生蛞蝓有关的线虫进行了调查。从 82 个地点共收集到 2406 条蛞蝓。蛞蝓被斩首，尸体被孵化两周，新出现的线虫被移除并处理以进行识别。使用 18S 核糖体 DNA 的分子序列数据鉴定线虫。在调查的 82 个地点中，有 20 个发现了线虫，其中 24.4% 的蛞蝓感染了线虫。共有七种线虫被鉴定到物种水平，包括秀丽隐杆线虫,Panagrolaimus papillosus,黄褐斑,伪圆藻,轴杆菌,无形弹状线虫和加州念珠菌. 另外四个标本被鉴定到属水平，包括奥舍乌斯sp。(9),Pristionchussp.,杆状炎sp。和弹珠菌素sp。(1)。两种最常见的线虫种类是秀丽隐杆线虫和P. pseudoteres. 兼性寄生虫，P. californica, 是从一个单一的红豆杉标本，采集自季节性苗圃。据我们所知，这项研究代表了加拿大对蛞蝓相关线虫的首次调查。