Klebsiella pneumoniae (Kp) is one of the most important etiological factors of urinary tract infections in renal transplant (RTx) recipients. We described the antimicrobial susceptibility phenotypes and genomic features of two hypermucoviscous (HM) Kp isolates recovered from RTx recipients with asymptomatic bacteriuria (ABU). Using whole genome sequencing (WGS) data, we showed that the strains belong to the ST152 lineage with the KL149 capsular serotype, but without rmpA/magA genes, which is typical for HM+ hypervirulent Kp. These new strains carried virulence-associated genes that predispose for urinary tract infections (UTIs). Likewise, both strains carried the ecp gene encoding pilus common for extended-spectrum β-lactamase (ESBL) Escherichia coli. Although the two ST152 isolates were closely related and differed by only nine single nucleotide polymorphisms (SNPs) in their chromosomes, they had different plasmid compositions and chromosomal elements, with isolate KP28872 carrying an ESBL plasmid and an integrative conjugative element. These two isolates are an example of the high plasticity of the K. pneumoniae accessory genome. The identification of patients with ABU matched with the correct epidemiological profiling of isolates could facilitate interventions to prevent or rapidly treat K. pneumoniae infections.

中文翻译：

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从无症状性菌尿肾移植受者中分离出的具有高粘液性表型的肺炎克雷伯菌 ST152 新变种——WGS 的遗传特征

肺炎克雷伯菌 (Kp) 是肾移植 (RTx) 受者尿路感染的最重要病因之一。我们描述了从无症状菌尿 (ABU) 的 RTx 接受者身上回收的两种高黏液 (HM) Kp 分离株的抗菌药敏感性表型和基因组特征。使用全基因组测序 (WGS) 数据，我们发现菌株属于 ST152 谱系，具有 KL149 荚膜血清型，但没有 rmpA/magA 基因，这是 HM+ 超毒力 Kp 的典型特征。这些新菌株携带易患尿路感染 (UTI) 的毒力相关基因。同样，这两种菌株都携带了编码超广谱 β-内酰胺酶 (ESBL) 大肠杆菌常见菌毛的 ecp 基因。尽管两个 ST152 分离株密切相关并且仅在染色体上有 9 个单核苷酸多态性 (SNP) 不同，但它们具有不同的质粒组成和染色体元件，分离株 KP28872 携带 ESBL 质粒和整合接合元件。这两个分离株是肺炎克雷伯氏菌附属基因组高可塑性的一个例子。ABU 患者的鉴定与正确的分离株流行病学特征相匹配，可以促进预防或快速治疗肺炎克雷伯菌感染的干预措施。肺炎辅助基因组。ABU 患者的鉴定与正确的分离株流行病学特征相匹配，可以促进预防或快速治疗肺炎克雷伯菌感染的干预措施。肺炎辅助基因组。ABU 患者的鉴定与正确的分离株流行病学特征相匹配，可以促进预防或快速治疗肺炎克雷伯菌感染的干预措施。