Reactive oxygen species (ROS) are generated during skin aging, including intrinsic (chronologic aging) and extrinsic aging (photoaging). Therefore, antioxidants that inhibit ROS generation can delay skin aging. In this study, we evaluated the potential anti-skin aging effect of (-)-phenolic compounds isolated from the root bark of Ulmus davidiana var. japonica. We preferentially investigated the possible preventive effects of isolates against the degradation of skin extracellular matrix. Among the isolates, (-)-catechin suppressed the activity of collagenase MMP-1, and reversed the degradation of collagen induced by tumor necrosis factor-α (TNF-α) in normal human dermal fibroblast. This action mechanism of (-)-catechin was validated by the suppression of tumor necrosis factor-α-induced accumulation of ROS and activation of mitogen-activated protein kinases, protein kinase B (Akt), and cyclooxygenase-2 (COX-2). The proinflammatory cytokines upregulate inflammatory reactions, and ultimately promote aging-related reactions. In this milieu, we demonstrated that (-)-catechin decreased the expression and secretion of proinflammatory cytokines, including interleukin (IL)-1β and IL-6. In conclusion, (-)-catechin is a candidate to ameliorate both intrinsic and extrinsic skin aging.

中文翻译：

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从榆树根皮中分离出（-）-儿茶素的抗皮肤老化作用 肿瘤坏死因子-α刺激的正常人皮肤成纤维细胞中的粳稻

活性氧（ROS）是在皮肤衰老过程中产生的，包括内在的（时间性衰老）和外在的性衰老（光老化）。因此，抑制ROS生成的抗氧化剂可以延缓皮肤衰老。在这项研究中，我们评估了从榆树根皮中分离出的（-）-酚类化合物的潜在抗皮肤衰老作用。粳稻。我们优先研究了分离物对皮肤细胞外基质降解的可能预防作用。在这些分离物中，（-）-儿茶素抑制了正常人真皮成纤维细胞中胶原酶MMP-1的活性，并逆转了由肿瘤坏死因子-α（TNF-α）诱导的胶原蛋白的降解。（-）-儿茶素的这种作用机制通过抑制肿瘤坏死因子-α诱导的ROS积累和丝裂原激活的蛋白激酶，蛋白激酶B（Akt）和环氧合酶2（COX-2）的激活而得到验证。促炎细胞因子上调炎症反应，并最终促进衰老相关反应。在此环境中，我们证明了（-）-儿茶素降低了促炎细胞因子（包括白介素（IL）-1β和IL-6）的表达和分泌。结论，