The bacterial candidate phyla radiation (CPR) and the archaeal DPANN superphylum are two novel lineages that have substantially expanded the tree of life due to their large phylogenetic diversity. Because of their ultrasmall size, reduced genome, and lack of core biosynthetic capabilities, most CPR and DPANN members are predicted to be sustained through their interactions with other species. How the few characterized CPR and DPANN symbionts achieve these critical interactions is, however, poorly understood. Here, we conducted an in silico analysis on 2,597 CPR/DPANN genomes to test whether these ultrasmall microorganisms might encode homologs of reference proteins involved in the synthesis and/or the detection of 26 different types of communication molecules (quorum sensing [QS] signals), since QS signals are well-known mediators of intra- and interorganismic relationships. We report the discovery of 5,693 variants of QS proteins distributed across 63 CPR and 6 DPANN phyla and associated with 14 distinct types of communication molecules, most of which were characterized as interspecies QS signals.

中文翻译：

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与种间和跨界交流相关的 CPR 和 DPANN 中群体感应蛋白编码序列的丰富库

细菌候选门辐射 (CPR) 和古细菌 DPANN 超门是两个新的谱系，由于其巨大的系统发育多样性，它们极大地扩展了生命之树。由于它们的超小尺寸、减少的基因组和缺乏核心生物合成能力，预计大多数 CPR 和 DPANN 成员将通过与其他物种的相互作用维持生命。然而，很少有人了解有特征的 CPR 和 DPANN 共生体如何实现这些关键的相互作用。在这里，我们进行了计算机模拟对 2,597 个 CPR/DPANN 基因组进行分析，以测试这些超小型微生物是否可能编码参与合成和/或检测 26 种不同类型通讯分子（群体感应 [QS] 信号）的参考蛋白的同源物，因为 QS 信号很好-已知的生物体内和生物间关系的中介。我们报告了 5,693 种 QS 蛋白变体的发现，它们分布在 63 个 CPR 和 6 个 DPANN 门中，并与 14 种不同类型的通信分子相关，其中大多数被表征为种间 QS 信号。