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Identification and molecular characterization of novel Rht‐1 alleles in hard red spring wheat
Crop Science ( IF 2.3 ) Pub Date : 2020-10-13 , DOI: 10.1002/csc2.20375
Emma M. Jobson 1 , John M. Martin 1 , Robert Sharrock 1 , Andrew C. Hogg 1 , Michael J. Giroux 1
Affiliation  

The introduction of the semidwarfing alleles, Rht‐B1b and Rht‐D1b, into wheat (Triticum aestivum L.) varieties significantly increased wheat yields beginning in the 1960s. Despite their agronomic advantage, Rht‐B1b and Rht‐D1b are also associated with decreased grain protein content and decreased coleoptile length. Furthermore, there is limited allelic diversity available to wheat breeders beyond Rht‐B1b and Rht‐D1b. We identified 16 ethyl methanesulfonate (EMS)‐induced Rht‐1 alleles, including a nonsense mutation in each Rht‐1 gene: Rht‐A‐Q555stop, Rht‐B‐Q420stop, and Rht‐D‐W559stop. We focused our attention for this study on stop alleles since the Rht‐B1b and Rht‐D1b alleles also contain premature stop codons. We evaluated the new nonsense alleles by measuring their impact on coleoptile length, gibberellic acid (GA) sensitivity, and DELLA/GID1 interaction. Rht‐A‐Q555stop reduced coleoptile length 16% compared with Rht‐1a (wild type) and repressed coleoptile GA perception similarly to Rht‐B1b and Rht‐D1b. Rht‐B‐Q420stop and Rht‐D‐W559stop did not affect coleoptile length. However, Rht‐B‐Q420stop reduced the ability of germinating seeds to perceive GA, but not as much as Rht‐A555stop, Rht‐B1b, or Rht‐D1b. We also tested whether the novel mutations affected the interaction between DELLA/GID1 in the presence and absence of GA. We found that Rht‐A‐Q555stop, Rht‐B‐Q420stop, and Rht‐D‐W559stop inhibited DELLA/GID1 interaction in the absence of GA, but the interaction was restored in the presence of GA. The identification of Rht‐A‐Q555stop, Rht‐B‐Q420stop, and Rht‐D‐W559stop increases the allelic diversity of Rht‐1. This study also indicates that the RHT carboxyl terminus is important for DELLA/GID1 interactions.

中文翻译:

硬红春小麦中新型Rht-1等位基因的鉴定与分子特征

半矮等位基因Rht-B1bRht-D1b引入小麦(Triticum aestivum L.)品种,从1960年代开始显着提高了小麦产量。尽管具有农艺优势,Rht-B1bRht-D1b还与谷物蛋白质含量降低和胚芽鞘长度降低有关。此外,除Rht-B1bRht-D1b之外,小麦育种者可利用的等位基因多样性有限。我们鉴定了16个由甲磺酸乙酯(EMS)诱导的Rht-1等位基因,包括每个Rht-1基因中的无意义突变:Rht-A-Q555stopRht-B-Q420stopRht‐D‐W559stop。由于Rht-B1bRht-D1b等位基因也包含过早的终止密码子,因此我们将注意力集中在终止等位基因上。我们通过测量新的无意义等位基因对胚芽鞘长度,赤霉素(GA)敏感性和DELLA / GID1相互作用的影响来评估。与Rht‐1a(野生型)相比,Rht‐A‐Q555stop的胚芽鞘长度减少了16%,并与Rht‐B1bRht‐D1b类似地抑制了胚芽鞘GA的感知。Rht-B-Q420stopRht-D-W559stop不会影响胚芽鞘的长度。但是,Rht-B-Q420stop降低了发芽种子感知GA的能力,但不如Rht-A555stopRht-B1bRht-D1b少。我们还测试了在存在和不存在GA的情况下,新型突变是否影响DELLA / GID1之间的相互作用。我们发现,在不存在GA的情况下,Rht-A-Q555stopRht-B-Q420stopRht-D-W559stop抑制了DELLA / GID1相互作用,但在存在GA的情况下,相互作用得以恢复。的识别RHT-A-Q555stopRHT-B-Q420stop,和RHT-d-W559stop增加的等位基因多样性RHT-1。该研究还表明,RHT羧基末端对于DELLA / GID1相互作用很重要。
更新日期:2020-10-13
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