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Expression and localization of adiponectin and its receptors (AdipoR1 and AdipoR2) in the hypothalamic-pituitary-ovarian axis of laying hens
Theriogenology ( IF 2.8 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.theriogenology.2020.10.020
Chong Li 1 , Qi Li 1 , Jing Li 1 , Na Zhang 1 , Yuanfang Li 1 , Yijie Li 1 , Hong Li 1 , Fengbin Yan 1 , Xiangtao Kang 1 , Xiaojun Liu 1 , Kui Li 1 , Yadong Tian 1
Affiliation  

Adiponectin is a hormone secreted by adipose tissue that is involved in the regulation of energy homeostasis and reproduction. In this study, the expression levels of adiponectin and its receptors in the hypothalamic-pituitary-ovarian (HPO) axis of laying hens were investigated using quantitative real-time PCR (qRT-PCR) and Western blotting, and the localization of these proteins was explored using immunohistochemistry. The morphological relationships between adiponectin receptors and gonadotropin-releasing hormone (GnRH) neurons were analyzed using double immunofluorescence labeling. The results showed that adiponectin mRNA and protein were widely expressed in all tissues involved in the HPO axis in laying hens, with especially high expression in the hypothalamus. Both AdipoR1 and AdipoR2 were more highly expressed in the pituitary than in other tissues and exhibited similar mRNA and protein expression patterns. The immunohistochemistry results showed that adiponectin and AdipoR2 were localized in the major hypothalamic nuclei that regulate food intake and energy balance (i.e., the lateral hypothalamic area (LHA), infundibular nucleus (IN), dorsomedial nucleus (DMN), and paraventricular nucleus (PVN)). Immunostaining revealed that adiponectin and its receptors were also localized in the cytoplasm of cells in the adenohypophysis. In the ovaries, adiponectin was localized in the granulosa layer, in the theca externa of follicles and in basal cells, while AdipoR1 and AdipoR2 were localized in basal cells. In the double immunofluorescence labeling experiment, AdipoR1 and AdipoR2 were localized in GnRH neurons in the IN and DMN. These results suggest that adiponectin and its receptors may play major roles in the endocrine network, which integrates energy balance and reproduction.

中文翻译:

蛋鸡下丘脑-垂体-卵巢轴中脂联素及其受体(AdipoR1和AdipoR2)的表达及定位

脂联素是脂肪组织分泌的一种激素,参与调节能量稳态和繁殖。本研究采用实时定量 PCR (qRT-PCR) 和蛋白质印迹法研究了蛋鸡下丘脑-垂体-卵巢 (HPO) 轴中脂联素及其受体的表达水平,这些蛋白的定位是使用免疫组织化学进行探索。使用双免疫荧光标记分析脂联素受体和促性腺激素释放激素 (GnRH) 神经元之间的形态关系。结果表明,脂联素mRNA和蛋白在蛋鸡HPO轴所涉及的所有组织中广泛表达,尤其是在下丘脑中表达量最高。AdipoR1 和 AdipoR2 在垂体中的表达高于其他组织,并表现出相似的 mRNA 和蛋白质表达模式。免疫组化结果表明,脂联素和 AdipoR2 定位于调节食物摄入和能量平衡的主要下丘脑核(即下丘脑外侧区 (LHA)、漏斗核 (IN)、背内侧核 (DMN) 和室旁核 (PVN) ))。免疫染色显示脂联素及其受体也位于腺垂体细胞的细胞质中。在卵巢中,脂联素位于颗粒层、卵泡外膜和基底细胞中,而 AdipoR1 和 AdipoR2 位于基底细胞中。在双重免疫荧光标记实验中,AdipoR1 和 AdipoR2 位于 IN 和 DMN 的 GnRH 神经元中。这些结果表明脂联素及其受体可能在内分泌网络中起主要作用,内分泌网络整合了能量平衡和繁殖。
更新日期:2021-01-01
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