Expression of transcription factors is crucial for the regulation of steroidogenesis and gonadal development in fish. SRY-related box (SOX) proteins regulate gene expression of various events related to vertebrate reproduction. This study reports the role of sox30 and its influence on sox9a/b in regulating testicular steroidogenesis of the common carp, Cyprinus carpio. Tissue distribution showed predominant expression of sox30 in gonads, while gonadal ontogeny indicated significant dimorphic expression of sox30 from 120 days post hatch. Higher sox30 transcripts during the spawning season, an elevation of sox30 after human chorionic gonadotropin induction, and 11-ketotestosterone (11-KT) treatment authenticate gonadotropin dependency. Treatment of 17α–methyl-di-hydroxy-testosterone to juvenile common carp for mono-sex induction, vis-à-vis elevated sox30 expression. Sox30 protein was detected abundantly in spermatocytes and spermatid/sperm of carp testis. Transient silencing of sox30 using small interfering RNAs decreased sox9a/b expression, lead to downregulation of certain molecule/factor, transcription factor, germ/stem cell marker, and steroidogenesis-related enzyme genes. Serum testosterone and 11-KT decreased significantly upon transient silencing of sox30, in vivo. Concomitantly, a reduction in testicular microsomal 11-β hydroxysteroid dehydrogenase activity was observed. These results demonstrate the influence of sox30 as well as sox9a/b in the regulation of testicular steroidogenesis in common carp.

转录因子的表达对于调节鱼类中的类固醇生成和性腺发育至关重要。SRY相关盒（SOX）蛋白调节与脊椎动物繁殖相关的各种事件的基因表达。这项研究报道了sox30在调节鲤鱼Cyprinus carpio睾丸类固醇生成中的作用及其对sox9a / b的影响。组织分布显示性腺中sox30的主要表达，而性腺个体发育表明从孵化后120天开始sox30的显着二态表达。产卵季节中较高的sox30成绩单，sox30的升高在人类绒毛膜促性腺激素诱导后，和11-酮睾酮（11-KT）治疗可验证促性腺激素依赖性。处理17α-甲基-二羟基睾丸激素对幼年鲤鱼的单性诱导（相对于sox30表达升高）。在鲤鱼睾丸的精细胞和精子/精子中大量检测到Sox30蛋白。使用小的干扰RNA对sox30进行瞬时沉默会降低sox9a / b的表达，从而导致某些分子/因子，转录因子，生殖/干细胞标记以及类固醇生成相关酶基因的下调。在体内短暂使Sox30沉默后，血清睾丸激素和11-KT明显降低。同时，观察到睾丸微粒体11-β羟类固醇脱氢酶活性降低。这些结果证明了sox30以及sox9a / b对鲤鱼睾丸类固醇生成的调节作用。