Lyme borreliosis has not been studied in Jordan or in much of the Middle East. However, limited research indicates that the tick vector, Ixodes ricinus, exists in the region. This study examined the seroprevalence of B. burgdorferi s.l. in Jordan and potential demographic and zoonotic risk factors for seropositivity. Serum samples of 824 apparently healthy participants from 11 governorates in Jordan were tested for B. burgdorferi s.l. using Enzygnost Lyme link VlsE/IgG enzyme-linked immunosorbent assay. A validated questionnaire was used to collect demographic and animal exposure data. Univariate and multivariate logistic regression were used to identify factors associated with seropositivity. The results showed that 11.7 % (95 % CI, 9.3–14.0 %) of the participants were seropositive for B. burgdorferi s.l.. There was a bimodal age distribution of seroprevalence with higher seroprevalence among individuals <20 and>60 years old. After controlling for governorate of residence, females had 2.77 (95 % CI 1.53–5.00) times greater odds of seropositivity compared to males. Individuals living in the southeastern part of Jordan (Ma’an) had 2.32 (95 % CI, 1.02−5.31) greater odds of seropositivity compared to those living in Amman, the Capital of Jordan, while those living in the northeast had significantly lower odds of seropositivity. This study presents the first evidence of B. burgdorferi s.l. seropositivity in Jordan and suggests several risk factors which were reported in studies conducted elsewhere. This study suggests that Lyme borreliosis should be considered in the differential diagnosis for patients presenting with skin lesions in Jordan.

在约旦或中东大部分地区尚未研究过莱姆病。但是，有限的研究表明，该区域存在壁虱媒介I虱（Ixodes ricinus）。这项研究检查了约旦地区B. burgdorferi sl的血清阳性率以及血清阳性的潜在人口统计学和人畜共患病危险因素。测试了约旦11个省的824名明显健康的参与者的血清样本中的伯氏疏螺旋体sl使用Enzygnost Lyme连接VlsE / IgG酶联免疫吸附测定。经过验证的调查表用于收集人口统计数据和动物暴露数据。单因素和多因素逻辑回归用于确定与血清阳性相关的因素。结果显示，有11.7％（95％CI，9.3-14.0％）的参与者血清B呈阳性。伯格多夫里sl。在20岁以上和60岁以上的人群中，血清阳性率存在双峰年龄分布，血清阳性率更高。在控制住所省份后，女性的阳性反应几率是男性的2.77（95％CI 1.53–5.00）倍。与居住在约旦首都安曼的人相比，居住在约旦（马安）东南部的人出现血清阳性的几率高2.32（95％CI，1.02-5.31）。血清阳性。这项研究提出了伯氏疏螺旋体的第一个证据。约旦的血清阳性，提示在其他地方进行的研究中报告了几种危险因素。这项研究表明，在约旦患有皮肤病变的患者的鉴别诊断中应考虑莱姆病的诊断。