The present contribution investigates the biocatalytic performance of the lipase obtained from the latex of the native plant known as Araujia sericifera (ASL) in the hydrolysis followed by the esterification of released free fatty acids and transesterification of glycerides of sunflower waste cooking oil WCO. A specific enzymatic activity of 719.05 µmol mg^-1 h^-1 (60 % conversion of triglycerides towards free fatty acids) was obtained in the hydrolysis of WCO with 0.05 % of biocatalyst (typically, 10.0 g WCO and 5.0 mg of enzyme) and 50 % w/w of water added in 30 min of reaction at 25 °C in homogeneous type of reaction system. The lipase has the capacity to catalyze the hydrolysis of the triglycerides mainly towards monoglycerides and diglycerides in a lesser extent. The released FFAs and the remaining glycerides reacted in tandem by addition of short chain alcohols immediately after the hydrolysis (without addition of more enzyme to the reaction media). Above 90 % conversion of the FFAs was obtained with methanol, ethanol, n-propanol and n-butanol at contents as low as 1:0.2 oil: alcohol molar ratio at 25 °C. The transesterification of the remaining monoglycerides (about 20 %) was also observed although no further reaction of the triglycerides was detected even under a great excess of alcohol.

本贡献调查了从称为Araujia sericifera（ASL）的天然植物的乳胶中获得的脂肪酶在水解，随后的游离脂肪酸的酯化和向日葵废食用油WCO的甘油酯的酯交换中的生物催化性能。比酶活性为719.05 µmol mg ^-1  h ^-1在0.05％的生物催化剂（通常为10.0 g WCO和5.0 mg的酶）和50％w / w的水在30℃的反应条件下加入WCO的水解过程中，获得了WCO（甘油三酸酯60％转化为游离脂肪酸）。在均相反应体系中为25°C。脂肪酶具有较小程度地主要催化甘油三酸酯向甘油单酸酯和甘油二酸酯水解的能力。水解后立即释放短链醇，使释放的FFA和剩余的甘油酯串联反应（不向反应介质中添加更多的酶）。用甲醇，乙醇，正丙醇和正丁醇在25°C下油与醇的摩尔比低至1：0.2时，FFA的转化率超过90％。