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Preparation, characterization and antioxidant activity of protocatechuic acid grafted carboxymethyl chitosan and its hydrogel
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2020-10-13 , DOI: 10.1016/j.carbpol.2020.117210
Chao Xu , Shui Guan , Jianqiang Xu , Weitao Gong , Tianqing Liu , Xuehu Ma , Changkai Sun

In this study, protocatechuic acid (PCA) was grafted onto carboxymethyl chitosan (CMCS) via EDC/NHS to improve the antioxidant effect. The grafting ratio of PCA-g-CMCS conjugates could be controlled by adjusting the pH value and feed ratio of raw materials. The conjugates exhibited similar pH sensitivity to CMCS and showed dramatic enhancements of DPPH and ABTS radicals scavenging activities, total antioxidant capacity, reducing power, and Fe2+-chelating activity. Three-dimensional porous PCA-g-CMCS hydrogel was prepared by lyophilization and secondary cross-linking. The shaped hydrogel preserved its antioxidant activity, and the sustained release of PCA-containing degraded fragment from biodegradable hydrogel could be achieved with the aid of lysozyme in vitro (15 days). PCA-g-CMCS hydrogel also showed excellent biocompatibility and protective effect on H2O2-induced oxidative damage in SH-SY5Y cells. These results suggested that PCA-g-CMCS conjugates and its hydrogel would appear to be a promising oxidation-resistant material for applications such as drug release and tissue engineering.



中文翻译:

原儿茶酸接枝羧甲基壳聚糖及其水凝胶的制备,表征及抗氧化活性

在这项研究中,原儿茶酸(PCA)通过EDC / NHS接枝到羧甲基壳聚糖(CMCS)以提高抗氧化作用。PCA-g-CMCS共轭物的接枝率可以通过调节原料的pH值和进料比来控制。共轭物显示出与CMCS相似的pH敏感性,并显示出DPPH和ABTS自由基清除活性,总抗氧化能力,还原能力和Fe 2+的显着增强螯合活动。通过冻干和二次交联制备三维多孔PCA-g-CMCS水凝胶。成型的水凝胶保留了其抗氧化活性,可以在体外(15天)借助溶菌酶实现从可生物降解的水凝胶中持续释放含PCA的降解片段。PCA-g-CMCS水凝胶在SH-SY5Y细胞中还表现出优异的生物相容性和对H 2 O 2诱导的氧化损伤的保护作用。这些结果表明,PCA-g-CMCS共轭物及其水凝胶似乎是一种有前途的抗氧化材料,可用于药物释放和组织工程等应用。

更新日期:2020-10-13
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