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Dimanganese trioxide (Mn2O3) based label-free electrochemical biosensor for detection of Aflatoxin-B1
Bioelectrochemistry ( IF 5 ) Pub Date : 2020-10-13 , DOI: 10.1016/j.bioelechem.2020.107684
Avinash Kumar Singh 1 , Tarun Kumar Dhiman 2 , Lakshmi G B V S 2 , Pratima R Solanki 2
Affiliation  

This work presents, a manganese oxide nanoparticles (Mn2O3nps) based electrochemical immunosensor for the detection of Aflatoxin-B1 (AFB1). X-ray diffrraction spectroscopy study confirms the purely synthesized Mn2O3nps with an average crystallite size of 31.5 nm. Transmission electron microscopy study confirms average particle size of 45 nm. To fabricate an electrochemical biosensor, a thin film of Mn2O3nps was fabricated onto indium tin oxide (ITO) surface using electrophoretic technique. Such fabricated thin film was utilized to immobilize antibodies (Anti-AFB1) for the selective detection of AFB1 using differential pulse voltammetry technique. Prior to perform sensing, bovine serum albumin (BSA) was utilized to block the uncovered sites on the Anti-AFB1/Mn2O3/ITO immunoelectrode surface. The response of BSA/Anti-AFB1/Mn2O3/ITO immunoelectrode was measured as a function of AFB1 in a linear detection range of 1 pg mL−1 to 10 µg mL−1 and sensor showed highest sensitivity of 2.044 μA mL ng−1cm−2 with lower detection limit of 0.54 pg mL−1. A spiked sample response of corn extract was studied in the linear range of 1 pg mL−1 to 10 µg mL−1 and immunoelectrode (BSA/Anti-AFB1/Mn2O3/ITO) showed recovery rate of 98.6 %.



中文翻译:

基于三氧化二锰(Mn 2 O 3)的无标记电化学生物传感器检测黄曲霉毒素B1

这项工作提出了一种基于锰氧化物纳米颗粒(Mn 2 O 3 nps)的电化学免疫传感器,用于检测黄曲霉毒素B1(AFB1)。X射线衍射光谱研究证实了纯合成的Mn 2 O 3 nps,其平均微晶尺寸为31.5 nm。透射电子显微镜研究证实平均粒径为45 nm。为了制造电化学生物传感器,需要制作Mn 2 O 3薄膜使用电泳技术将nps制造在氧化铟锡(ITO)表面上。使用这种制造的薄膜来固定抗体(Anti-AFB1),以使用差分脉冲伏安技术选择性检测AFB1。在进行感测之前,使用牛血清白蛋白(BSA)封闭抗AFB1 / Mn 2 O 3 / ITO免疫电极表面上未发现的位点。BSA /反AFB1的/响应的Mn 2 ö 3 / ITO immunoelectrode测量为AFB 1的函数在1微克mL的线性检测范围-1至10μg毫升-1和传感器表明2.044μA毫升纳克的灵敏度最高-1厘米-2最低检测限为0.54 pg mL -1。在1 pg mL -1至10 µg mL -1的线性范围内研究了玉米提取物的加标样品响应,免疫电极(BSA / Anti-AFB1 / Mn 2 O 3 / ITO)的回收率为98.6%。

更新日期:2020-10-30
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