The optimal mixed feeding schedule for mono-sex male Nile tilapia (Oreochromis niloticus) was investigated in the current study. The fish (4.01 ± 0.02 g initial weight and 5.84 ± 0.04 cm initial length) were fed up to satiation with high protein (HP, 32.80 % protein on dry basis) and low protein (LP, 28.64 % protein on dry basis) diets in different schedules: alternately every other day (1HP/1 L P), alternately every other week (7HP/7 L P) and alternately every two weeks (14HP/14 L P), in comparison to continuously feeding by HP (positive control) or by LP (negative control) alone. At the end of eight weeks of trial, superior growth performance and feed utilization were observed in fish receiving HP, 1HP/1 L P and 7HP/7 L P relative to the remaining treatments. The specific activity of digestive enzymes (pepsin, trypsin, amylase, lipase, and activity ratio of amylase to trypsin) was modulated, indicating physiological responses for improving nutrient utilization due to the different feeding regimens. All fish mainly utilized carbohydrates rather than lipid and protein, or probably the protein-sparing effect was predominant, as indicated by the positive correlation between amylase specific activity and protein efficiency ratio (r = 0.681, P < 0.01), across five feeding schedules. There were no negative effects of mixed feeding schedules on flesh quality and carcass composition in any experimental group. However, based on economic analysis, fish receiving 1HP/1LP schedule had reduced cost of tilapia production relative to the other treatments. Therefore, the optimal feeding schedule for mono-sex male tilapia is 1HP/1LP, which should be used as a practical feeding guideline for mono-sex male Nile tilapia production.

单性雄性尼罗罗非鱼（Oreochromis niloticus）在当前研究中进行了调查。鱼（初始重量为4.01±0.02 g，初始长度为5.84±0.04 cm）以高蛋白（HP，干重为32.80％蛋白质）和低蛋白（LP，干重为28.64％蛋白质）的饲料喂养。不同的时间表：与HP（阳性对照）或LP连续喂养相比，每隔一天（1HP / 1 LP），每隔一周（7HP / 7 LP）和每两周（14HP / 14 LP）交替（阴性对照）。在试验的八周结束时，与其余处理相比，在接受HP，1HP / 1 LP和7HP / 7 LP的鱼中观察到了优异的生长性能和饲料利用率。调节消化酶的比活（胃蛋白酶，胰蛋白酶，淀粉酶，脂肪酶以及淀粉酶与胰蛋白酶的活性比），表示由于不同的喂养方式而提高了养分利用率的生理反应。所有的鱼类主要利用碳水化合物而不是脂质和蛋白质，或者可能主要是蛋白质保留作用，如淀粉酶比活性和蛋白质效率比之间呈正相关（在五个饲喂计划中，r = 0.681，P <0.01）。在任何实验组中，混合饲喂日程对肉品质和car体组成均无负面影响。但是，基于经济分析，接受1HP / 1LP时间表的鱼比其他处理方法降低了罗非鱼的生产成本。因此，单性雄性罗非鱼的最佳饲喂时间表为1HP / 1LP，应作为单性雄性尼罗罗非鱼生产的实用饲喂准则。