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Evaluation of branched chain amino acids in male Cobb MV × 500 broiler chickens by using Box-Behnken response surface design
Animal Feed Science and Technology ( IF 3.2 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.anifeedsci.2020.114710
C.W. Maynard , S.Y. Liu , J.T. Lee , J.V. Caldas , J.J.E. Diehl , S.J. Rochell , M.T. Kidd

Abstract Data pertaining to the branched-chain amino acids (BCAA) are of particular importance due to the positioning of valine (Val) and isoleucine (Ile) as limiting amino acids (AA) in conventional diets. Similarly, current research evaluating their potential interaction is sparse. Therefore, two experiments were conducted using Box-Behnken design (BBD) to determine performance and carcass responses to BCAA and glycine (Gly) supplementation in male broilers from 15 to 34 days (Experiment 1) and 15 to 35 days (Experiment 2). In Experiment 1, 13 experimental diets were formulated to contain various levels of digestible BCAA supplementation based on BBD. Inclusion levels for the BCAA were based on ratios to digestible lysine (Lys) and included: 65, 75, and 85 for digestible Val; 58, 66, and 74 for digestible Ile; and 110, 130, and 150 for digestible leucine (Leu). Diets for Experiment 2 were formulated in a similar manner and design, but total glycine + serine (Gly + Ser) inclusions were substituted for Leu. Inclusion levels were the same for Val and Ile as in Experiment 1 and ratios to digestible Lys of 131, 151, and 171 for total Gly + Ser. Fifteen-hundred and sixty male broilers were distributed across 78 pens and randomly assigned to 13 experimental diets in each Experiment. Body weight (BW) gain, feed intake, and feed conversion ratio (FCR) were determined for the experimental grower period in both Experiments. On day 35 (Experiment 1) or 36 (Experiment 2), six birds per pen were processed for the evaluation of carcass traits. Data were analyzed for linear, quadratic, and interactive effects. In Experiment 1, increased Leu inclusion linearly increased (P

中文翻译:

使用Box-Behnken响应面设计评价雄性Cobb MV×500肉鸡支链氨基酸

摘要 由于缬氨酸 (Val) 和异亮氨酸 (Ile) 在常规饮食中被定位为限制性氨基酸 (AA),因此有关支链氨基酸 (BCAA) 的数据尤为重要。同样,目前评估它们潜在相互作用的研究很少。因此,使用 Box-Behnken 设计 (BBD) 进行了两个实验,以确定雄性肉鸡在 15 至 34 天(实验 1)和 15 至 35 天(实验 2)中对添加 BCAA 和甘氨酸 (Gly) 的性能和胴体反应。在实验 1 中,根据 BBD 配制了 13 种实验饮食,以包含不同水平的可消化 BCAA 补充剂。BCAA 的包含水平基于与可消化赖氨酸 (Lys) 的比率,包括: 65、75 和 85 可消化 Val;可消化的 Ile 为 58、66 和 74;和 110, 130, 可消化亮氨酸 (Leu) 为 150。实验 2 的饮食以类似的方式和设计配制,但总甘氨酸 + 丝氨酸 (Gly + Ser) 夹杂物被 Leu 替代。Val 和 Ile 的包含水平与实验 1 中的相同,总 Gly + Ser 与可消化赖氨酸的比率为 131、151 和 171。1560 只雄性肉鸡分布在 78 个围栏,并在每个实验中随机分配到 13 个实验日粮。在两个实验中都确定了实验生长期的体重 (BW) 增重、采食量和饲料转化率 (FCR)。在第 35 天(实验 1)或第 36 天(实验 2),每个围栏处理 6 只鸡以评估胴体特征。分析数据的线性、二次和交互效应。在实验 1 中,增加的 Leu 包含线性增加(P
更新日期:2021-01-01
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