In this work, two Co(II)-based coordination polymers with the chemical formulae [CoCl(cptpy)·2H2O]n (1) and [Co2Cl2(cptpy)2(ma)H2O]n (2, Hcptpy = 4′-(4-carboxyphenyl)-2,2′:6′,2″-terpyridine, ma = melamine) are hydrothermally synthesized without or with employment of the template melamine. The as-prepared two complexes are studied via single crystal X-ray diffraction along with the elemental analysis. Furthermore, the protective ability of the compounds on the osteoblast differentiation is evaluated and the related detailed mechanism is explored. Firstly, the Western blot is used to detect the activation of the Wnt/β-catenin signaling pathway after treatment with the compounds. Next, the RT-PCR assay is used to measure the relative expression of indicators of vegf and vwf angiogenesis.

中文翻译：

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两种基于 Co(II) 的配位聚合物：通过增加 WNT/β-catenin 信号通路激活对成骨细胞分化的结构洞察和刺激活性

在这项工作中，两种基于 Co(II) 的配位聚合物具有化学式 [CoCl(cptpy)·2H2O]n (1) 和 [Co2Cl2(cptpy)2(ma)H2O]n (2, Hcptpy = 4'- (4-羧基苯基)-2,2':6',2"-三联吡啶，ma = 三聚氰胺)在不使用或使用模板三聚氰胺的情况下水热合成。通过单晶 X 射线衍射和元素分析研究制备的两种配合物。此外，还评估了化合物对成骨细胞分化的保护能力，并探讨了相关的详细机制。首先，Western印迹用于检测化合物处理后Wnt/β-catenin信号通路的激活。接下来，RT-PCR 测定用于测量 vegf 和 vwf 血管生成指标的相对表达。