Pepper mild mottle virus (PMMoV), a tobamovirus, has been reported worldwide infecting, especially, pepper plants. In this study, seven new PMMoV isolates were identified in symptomatic pepper plants collected in four departments of Peru. The majority of these isolates induced severe symptoms, including mottle, in susceptible pepper plants. Inoculation on pepper plants carrying four known functional L resistance alleles (L1, L2, L3, and L4) revealed that PMMoV isolates from Peru belong to the P1,2 pathotype. Therefore, pepper species or cultivars with L3 and L4 alleles displayed hypersensitive response after mechanical inoculation and were still resistant to PMMoV isolates circulating in Peru. Infection by PMMoV was monitored by serology (DAS-ELISA) and RT-PCR with degenerate primers. Virus genes were used to build a phylogenetic tree that showed diversity accumulation among the PMMoV isolates from Peru.

中文翻译：

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来自秘鲁的辣椒轻度斑驳病毒分离株在易感辣椒植物中引起严重症状，属于 P1,2 致病型

辣椒轻度斑驳病毒 (PMMoV) 是一种烟草病毒，据报道在世界范围内感染，尤其是辣椒植物。在这项研究中，在秘鲁四个省收集的有症状的辣椒植物中发现了 7 个新的 PMMoV 分离株。这些分离株中的大多数在易感辣椒植物中引起严重的症状，包括斑纹。对携带四个已知功能性 L 抗性等位基因（L1、L2、L3 和 L4）的辣椒植物进行接种表明，来自秘鲁的 PMMoV 分离株属于 P1,2 致病型。因此，具有 L3 和 L4 等位基因的辣椒物种或栽培品种在机械接种后表现出超敏反应，并且仍然对秘鲁流行的 PMMoV 分离株具有抗性。PMMoV 感染通过血清学 (DAS-ELISA) 和使用简并引物的 RT-PCR 监测。