Molecular Genetics and Genomics ( IF 3.1 ) Pub Date : 2020-10-13 , DOI: 10.1007/s00438-020-01733-2 Abdelraheem Abdelraheem , Gregory N. Thyssen , David D. Fang , Johnie N. Jenkins , Jack C. McCarty , Tom Wedegaertner , Jinfa Zhang
Cotton is grown in arid and semi-arid regions where abiotic stresses such as drought and salt are prevalent. There is a lack of studies that simultaneously address the genetic and genomic basis of tolerance to drought and salt stress. In this study, a multi-parent advanced generation inter-cross (MAGIC) population of 550 recombinant inbred lines (RILs) together with their 11 Upland cotton parents with a total of 473,516 polymorphic SNP markers was used to identify quantitative trait loci (QTL) for drought tolerance (DT) and salt tolerance (ST) at the seedling stage based on two replicated greenhouse tests. Transgressive segregation occurred in the MAGIC-RILs, indicating that tolerant and sensitive alleles recombined for tolerance to the abiotic stress during the intermating process for the population development. A total of 20 QTL were detected for DT including 13 and 7 QTL based on plant height (PH) and dry shoot weight (DSW), respectively; and 23 QTL were detected for ST including 12 and 11 QTL for PH and DSW, respectively. There were several chromosomes with QTL clusters for abiotic stress tolerance including four QTL on chromosome A13 and three QTL on A01 for DT, and four QTL on D08 and three QTL on A11 for ST. Nine QTL (21% of the 43 QTL) detected were in common between DT and ST, indicating a common genetic basis for DT and ST. The narrow chromosomal regions for most of the QTL detected in this study allowed identification of 53 candidate genes associated with responses to salt and drought stress and abiotic stimulus. The QTL identified for both DT and ST have significantly augmented the repertoire of QTL for abiotic stress tolerance that can be used for marker-assisted selection to develop cultivars with resilience to drought and/or salt and further genomic studies towards the identification of drought and salt tolerance genes in cotton.
中文翻译:
GWAS揭示了来自550个品系的MAGIC群体的抗旱和耐盐性QTL,该群体来自11个陆地棉(陆地棉)亲本的确定
棉花生长在干旱和半干旱地区,那里普遍存在非生物胁迫,例如干旱和盐分。缺乏同时解决干旱和盐胁迫耐受性的遗传和基因组基础的研究。在这项研究中,使用550个重组自交系(RILs)及其11个陆地棉亲本以及总共473,516个多态性SNP标记的多亲代杂交(MAGIC)群体来鉴定数量性状基因座(QTL)根据两次重复的温室测试,确定苗期的耐旱性(DT)和耐盐性(ST)。在MAGIC-RIL中发生过分的分离,表明在种群发育的决定性过程中,耐受性和敏感等位基因重组了对非生物胁迫的耐受性。根据植物高度(PH)和干梢重量(DSW)分别检测到20个QTL,包括13和7个QTL;ST检测到23个QTL,PH和DSW检测到12个QTL。有数个具有非生物胁迫耐受性的QTL簇的染色体,其中DT的A13染色体上有四个QTL,DT的A01上有三个QTL,ST的D08上的四个QTL和A11上的三个QTL。检测到的9个QTL(占43个QTL的21%)在DT和ST之间共有,这表明DT和ST具有共同的遗传基础。在这项研究中检测到的大多数QTL,其狭窄的染色体区域可以鉴定与盐和干旱胁迫以及非生物刺激反应相关的53个候选基因。