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LED Lights Promote Growth and Flavonoid Accumulation of Anoectochilus roxburghii and Are Linked to the Enhanced Expression of Several Related Genes
Plants ( IF 4.658 ) Pub Date : 2020-10-12 , DOI: 10.3390/plants9101344
Do Thi Gam , Phan Hong Khoi , Pham Bich Ngoc , Ly Khanh Linh , Nguyen Khac Hung , Phan Thi Lan Anh , Nguyen Thi Thu , Nguyen Thi Thu Hien , Tran Dang Khanh , Chu Hoang Ha

Anoectochilus roxburghii is a wild edible species and has been traditionally used for a wide range of diseases in many countries. Our research aims to find the optimal light-emitting diode (LED) lighting conditions to improve the growth and development of A. roxburghii seedling at the acclimation stage. Two-month-old explants were cultured under the various lighting conditions including red (R), blue (B), BR (one blue: four red), BRW151 (one blue: five red: one white), BRW142 (one blue: four red: two white), and fluorescent lamp (FL). The results showed that the lighting conditions not only affect the growth and morphology of plants but also the accumulation of total flavonoids. Single wavelengths (B or R LED) inhibited the growth and secondary biosynthesis of A. roxburghii, while the BR LED showed an enhancement in both growth and biomass accumulation. A. roxburghii plants were grown under BR LED light has average plant height (7.18 cm), stem diameter (17.6mm), number of leaves (5.78 leaves/tree), leaf area (4.67 cm2), fresh weight (0.459 g/tree), dry matter percentages (11.69%), and total flavonoid (1.811 mg/g FW) is considered to be superior to FL lamps and other LEDs in the experiment. This indicates that both blue and red wavelengths are required for the normal growth of A. roxburghii. To learn more about how light affects flavonoid biosynthesis, we evaluated the expression of genes involved in this process (pal, chs, chi, and fls) and found that BR LED light enhances the expression level of chi and fls genes compared to fluorescent lamps (1.18 and 1.21 times, respectively), leading to an increase in the flavonoid content of plant. Therefore, applying BR LED during in vitro propagation of A. roxburghii could be a feasible way to improve the medicinal value of this plant.

中文翻译:

LED灯促进非洲象鼻线虫的生长和类黄酮积累,并与几个相关基因的增强表达有关

roxburghii Anoectochilus roxburghii是一种野生食用物种,在许多国家传统上已用于多种疾病。我们的研究旨在找到最佳的发光二极管(LED)照明条件,以在适应阶段改善roxburghii幼苗的生长和发育。在两个不同的光照条件下培养了两个月大的外植体,包括红色(R),蓝色(B),BR(一个蓝色:四个红色),BRW151(一个蓝色:五个红色:一个白色),BRW142(一个蓝色:四个红色:两个白色)和荧光灯(FL)。结果表明,光照条件不仅影响植物的生长和形态,而且还影响总黄酮的积累。单波长(B或R LED)抑制了A. roxburghii的生长和次级生物合成,而BR LED的生长和生物量积累均增强。A. roxburghii植物在BR LED灯下生长,平均植物高度(7.18 cm),茎直径(17.6mm),叶片数(5.78叶片/树),叶面积(4.67 cm 2),鲜重(0.459 g /树),干物质百分比(11.69%)和总黄酮(1.811 mg / g FW)被认为在实验中优于FL灯和其他LED。这表明蓝光和红光波长对于拟南芥的正常生长都是必需的。要了解有关光如何影响类黄酮生物合成的更多信息,我们评估了此过程中涉及的基因(palchschifls)的表达。),发现BR LED光与荧光灯相比提高了chifls基因的表达水平(分别为1.18和1.21倍),从而导致植物中类黄酮含量的增加。因此,在拟南芥体外繁殖中应用BR LED可能是提高该植物药用价值的可行途径。
更新日期:2020-10-12
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