The zebrafish (Danio rerio) is used as an embryonic and larval model to perform in vitro experiments and developmental toxicity studies. Zebrafish may be used to determine the toxicity of samples in early screening assays, often in a high-throughput manner. The zebrafish embryotoxicity model is at the leading edge of toxicology research due to the short time required for analyses, transparency of embryos, short life cycle, high fertility, and genetic data similarity. Zebrafish toxicity studies range from assessing the toxicity of bioactive compounds or crude extracts from plants to determining the optimal process. Most of the studied extracts were polar, such as ethanol, methanol, and aqueous solutions, which were used to detect the toxicity and bioactivity. This review examines the latest research using zebrafish as a study model and highlights its power as a tool for detecting toxicity of medicinal plants and its effectiveness at enhancing the understanding of new drug generation. The goal of this review was to develop a link to ethnopharmacological zebrafish studies that can be used by other researchers to conduct future research.

中文翻译：

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斑马鱼作为筛选药用植物毒性的成功动物模型

斑马鱼（Danio rerio）被用作胚胎和幼虫模型来进行体外实验和发育毒性研究。斑马鱼通常可以以高通量的方式用于早期筛选测定中确定样品的毒性。由于分析所需的时间短，胚胎的透明性，生命周期短，高繁殖力和遗传数据相似性，斑马鱼胚胎毒性模型处于毒理学研究的前沿。斑马鱼的毒性研究范围从评估生物活性化合物或植物粗提物的毒性到确定最佳工艺。大多数研究提取物是极性的，例如乙醇，甲醇和水溶液，可用于检测毒性和生物活性。这篇综述审查了使用斑马鱼作为研究模型的最新研究，并着重介绍了斑马鱼作为检测药用植物毒性的工具及其在增强对新药产生的理解方面的效力的功能。这篇综述的目的是建立与民族药理斑马鱼研究的联系，其他研究人员可以将其用于进行未来的研究。