Agave sisalana agro-industrial residue has considerable potential against damage associated with oxidative stress and skin aging. This study aims to demonstrate, in vitro and in vivo, the potential of Agave sisalana agro-industrial residue as a safe and effective alternative for the prevention of damage caused by oxidative stress and aging. The antioxidant activity was evaluated in vitro (total antioxidant capacity, reducing power, DPPH radical scavenging, metal chelating (Fe²⁺ and Cu²⁺), and hydroxyl radical scavenging) and in vivo using the Caenorhabditis elegans organism model. The extract showed in vitro antioxidant activity in all tests performed. Tests with C. elegans showed that the extract was able to reduce the intracellular levels of reactive oxygen species (ROS) and increase the survival rate of worms. A downregulation of gst-4::GFP expression suggests a direct action against free radicals. Agave sisalana agro-industrial residue extract (AsRE) can therefore be considered as a source of antioxidant biomolecules, and the use of this agro-industrial residue in a new production process can lead to sustainability and socioeconomic development.

中文翻译：

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龙舌兰农业工业残留物的体外和体内抗氧化活性

龙舌兰 sisalana农业工业残留物具有相当大的潜力，可以防止与氧化应激和皮肤老化相关的损伤。本研究旨在在体外和体内证明龙舌兰农业工业残留物作为一种安全有效的替代品来预防氧化应激和衰老造成的损害的潜力。使用秀丽隐杆线虫生物模型在体外（总抗氧化能力、还原能力、DPPH 自由基清除、金属螯合（Fe ²⁺和 Cu ²⁺）和羟基自由基清除）和体内评估抗氧化活性。该提取物在进行的所有测试中均显示出体外抗氧化活性。线虫测试表明该提取物能够降低细胞内活性氧 (ROS) 的水平并提高蠕虫的存活率。的下调GST-4 :: GFP表达表明对抗自由基的直接作用。因此，龙舌兰农业工业残留物提取物 (AsRE) 可被视为抗氧化生物分子的来源，在新的生产过程中使用这种农业工业残留物可以促进可持续性和社会经济发展。