Local heating using pulsed-laser-induced photothermal effects on plasmonic nanostructured substrates can be used for intracellular delivery applications. However, the fabrication of plasmonic nanostructured interfaces is hampered by complex nanomanufacturing schemes. Here, we demonstrate the fabrication of large-area plasmonic gold (Au) nanodisk arrays that enable photothermal intracellular delivery of biomolecular cargo at high efficiency. The Au nanodisks (350 nm in diameter) were fabricated using chemical lift-off lithography (CLL). Nanosecond laser pulses were used to excite the plasmonic nanostructures, thereby generating transient pores at the outer membranes of targeted cells that enable the delivery of biomolecules via diffusion. Delivery efficiencies of >98% were achieved using the cell impermeable dye calcein (0.6 kDa) as a model payload, while maintaining cell viabilities at >98%. The highly efficient intracellular delivery approach demonstrated in this work will facilitate translational studies targeting molecular screening and drug testing that bridge laboratory and clinical investigations.

中文翻译：

---

使用金纳米盘阵列进行光热细胞内递送

使用脉冲激光诱导的光热效应对等离子体纳米结构基底进行局部加热可用于细胞内递送应用。然而，等离子体纳米结构界面的制造受到复杂的纳米制造方案的阻碍。在这里，我们展示了大面积等离子体金 (Au) 纳米盘阵列的制造，该阵列能够高效地光热细胞内递送生物分子货物。Au纳米盘（直径350纳米）是使用化学剥离光刻（CLL）制造的。纳秒激光脉冲用于激发等离子体纳米结构，从而在靶细胞的外膜上产生瞬时孔，从而能够通过扩散传递生物分子。使用细胞不可渗透染料钙黄绿素 (0. 6 kDa) 作为模型有效载荷，同时将细胞活力保持在 > 98%。这项工作中展示的高效细胞内递送方法将促进针对分子筛选和药物测试的转化研究，从而将实验室和临床研究联系起来。