Abstract Strain CG-9, isolated from human saliva and identified as Enterococcus faecalis, produced a component that inhibits the growth of Gram-positive and Gram-negative bacteria. The active peptide from the cell-free supernatant of Enterococcus faecalis CG-9 was purified in three steps: (1) precipitation with 70% saturated ammonium sulfate: (2) cation-exchange chromatography, (3) gel filter chromatography. The purified bacteriocin exhibited a wide range of antimicrobial activity against many food-borne spoilage and Pathogenic bacteria such as Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, as well as some yeast. The bacteriocin was heat stable, pH resistant and protease sensitive. Ethylenediaminetetraacetic acid, sodium dodecyl sulfate, Tween 80 and 6% sodium chloride did not decrease the activity. The characterization, antimicrobial spectrum and molecular mass of this bacteriocin suggested that it was a novel bacteriocin with potential research and application value in food industry.

中文翻译：

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粪肠球菌CG-9从人唾液中产生的新型细菌素的纯化和表征

摘要 从人类唾液中分离出的菌株 CG-9 被鉴定为粪肠球菌，它产生了一种抑制革兰氏阳性菌和革兰氏阴性菌生长的成分。来自粪肠球菌 CG-9 无细胞上清液的活性肽分三步纯化：(1) 用 70% 饱和硫酸铵沉淀：(2) 阳离子交换层析，(3) 凝胶过滤层析。纯化的细菌素对许多食源性腐败菌和致病菌表现出广泛的抗菌活性，如大肠杆菌、单核细胞增生李斯特菌、金黄色葡萄球菌以及一些酵母菌。细菌素是热稳定的、pH 抗性和蛋白酶敏感的。乙二胺四乙酸、十二烷基硫酸钠、吐温 80 和 6% 氯化钠没有降低活性。表征，