Abstract

Rice sheath blight, caused by Rhizoctonia solani, is one of the increasing concerns in rice causing severe economic losses worldwide. Chemical control measures are already in practice for the management of this disease but approach for discovery of new chemicals with improved disease control potential is essential. The present investigation involves the development of nanoformulations of halogen substituted azomethines as new potent fungicide for the management of sheath blight disease in rice. The halogen substituted azomethines were synthesized and screened for in-vitro bioefficacy. Then the azomethines were nanoformulated for their in-vivo pot culture evaluation against Rhizoctonia solani. Transmission electron microscopy confirmed the particle size of the developed nanoformulations in the range of 1–100 nm. In-vitro inhibition results of test compounds revealed their ED₅₀ values in the range of 11.9 to 22.4 μg/ml with best performer 2,4,5-trichloro-N-(2,4-dichlorobenzylidene)aniline. 3-Chloro-N-(2,6-dichlorobenzylidene)-4-fluoroaniline displayed maximum in-vivo efficacy against R. Solani which was comparable with that of hexaconazole, a standard recommended fungicide.

摘要

由立枯丝核菌引起的水稻纹枯病是水稻日益受到关注的问题之一，在全球范围内造成严重的经济损失。化学控制措施已经在实践中用于管理这种疾病，但发现具有改善疾病控制潜力的新化学物质的方法是必不可少的。目前的研究涉及开发卤素取代的偶氮甲碱纳米制剂作为治疗水稻纹枯病的新型强效杀菌剂。合成了卤素取代的偶氮甲碱并筛选了体外生物功效。然后将偶氮甲碱纳米制剂用于其针对立枯丝核菌的体内盆栽培养评估. 透射电子显微镜证实开发的纳米制剂的粒径在 1-100 nm 范围内。测试化合物的体外抑制结果显示其 ED ₅₀值在 11.9 至 22.4 μg/ml 的范围内，其中表现最好的 2,4,5-三氯-N-(2,4-二氯亚苄基)苯胺。3-Chloro-N-(2,6-dichlorobenzylidene)-4-fluoroaniline对R. Solani表现出最大的体内效力，与标准推荐的杀真菌剂 hexaconazole 相当。