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DNA microsatellite genotyping of potentially pathogenic Candida albicans and C. dubliniensis isolated from the oral cavity and dental prostheses
Microbial Pathogenesis ( IF 3.8 ) Pub Date : 2020-10-12 , DOI: 10.1016/j.micpath.2020.104548
Manoel Francisco Rodrigues Netto 1 , Jeferson Júnior da Silva 1 , Thaísla Andrielle da Silva 1 , Mateus Cardoso Oliveira 1 , José Francisco Höfling 1 , Eduardo de Andrade Bressan 2 , Antonio Vargas de Oliveira Figueira 2 , Marcelo Fabiano Gomes Boriollo 1
Affiliation  

This study investigated the incidence, genetic diversity, antifungal sensitivity, and virulence of Candida albicans and C. dubliniensis isolated from subjects using dental prostheses and subjects clinically indicated for the first prosthetic rehabilitation. Subjects were divided into four groups and samples were collected twice: at first rehabilitation by removable partial (A) and total (C) dental prostheses, and replacement of the removable partial (B) and total (D) prostheses. Yeasts were genotyped using DNA microsatellite markers. Microbiological methods were used to screen for azole antifungal resistance and exoenzyme production. In the initial sampling, oral colonization by Candida was observed in 31 (53.4%) subjects in groups A (33.3%), B (68.2%), and D (65%); 20 (47.6%) subjects displayed colonization of prostheses: groups B (50%) and D (45%). The second sampling (±30 days) revealed Candida in 2 (3.4%: oral cavity) and 4 (6.9%: prosthetic) subjects from group B. C. albicans and C. dubliniensis displayed both polyclonal and monoclonal patterns of infection. Azole-resistant C. albicans and SAPs+ strains were prevalent. Related strains were found in one or several oral sites (mucosa and prosthesis), as well as intra- and inter-subject, -gender, -group, and –time of sampling. However, the patterns of clonality can be altered under dental care.



中文翻译:

从口腔和义齿中分离出潜在致病性白色念珠菌杜氏念珠菌的DNA微卫星基因分型

这项研究调查了使用假牙和临床上首次进行假肢修复的受试者从受试者中分离出的白色念珠菌杜氏念珠菌的发病率,遗传多样性,抗真菌敏感性和毒力。将受试者分为四组,并收集两次样品:首先通过可移动的部分(A)和全部(C)假体进行康复,然后替换可移动的部分(B)和全部(D)假体。使用DNA微卫星标记对酵母进行基因分型。微生物学方法用于筛选唑类抗真菌药的耐药性和外酶的产生。在最初的采样中,念珠菌的口腔定植在A组(33.3%),B(68.2%)和D(65%)的31位受试者(53.4%)中观察到了这一现象;20名(47.6%)受试者表现出假体定植:B组(50%)和D组(45%)。第二采样(±30天)显示念珠菌和4(6.9%:假体)从组B.受试者:在2(口腔3.4%)白色念珠菌C.都柏林显示感染的多克隆抗体和单克隆图案。耐偶氮菌的白色念珠菌和SAPs +菌株普遍存在。在一个或几个口腔部位(粘膜和假体)以及受试者内和受试者间,性别,组和取样时间发现了相关菌株。但是,在牙科护理下,可以改变克隆模式。

更新日期:2020-10-15
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