A 56-day feeding trial was conducted to investigate the effects of dietary supplementation of Astragalus membranaceus or/and Bupleurum chinense on the growth performance, immune enzymes, and related gene expression of Pacific white shrimp (Litopenaeus vanammei). Six experimental diets were formulated and supplemented with two levels (0.25% and 0.5%) of each herb and their combination. At the end of the trial, the specific growth rate and feed conversion ratio of shrimp were significantly (P < 0.05) improved by herbal diets. Besides, the activities of immune-related enzymes such as superoxide dismutase (SOD), alkaline phosphatase (AKP), and lysozyme in serum and hepatopancreas were significantly (P < 0.05) elevated in shrimp fed A. membranaceus or/and B. chinense. The high expression levels of immune deficiency (IMD), lysozyme, and Toll-like receptor mRNA directly or indirectly reflected the activation effect of innate immune in shrimp by dietary A. membranaceus or/and B. chinense. However, no significant difference (P > 0.05) among the herbal incorporated treatments was detected on the growth performance and immune response. In conclusion, the results suggest that A. membranaceus and B. chinense could be used as a beneficial feed additives and alternatives to antibiotics for white shrimp aquaculture.

进行了为期56天的喂养试验，以研究日粮黄芪或/和柴胡对太平洋白虾（Litopenaeus vanammei）的生长性能，免疫酶和相关基因表达的影响。配制了六种实验饮食，并分别添加了两种含量（0.25％和0.5％）的每种草药及其组合。试验结束时 ，草药饮食显着改善了虾的比生长速度和饲料转化率（P <0.05）。此外，血清和肝胰腺中超氧化物歧化酶（SOD），碱性磷酸酶（AKP）和溶菌酶等免疫相关酶的活性显着（P （<0.05）在饲喂虾的A. membranaceus或/和B. chinense中升高。免疫缺陷（IMD），溶菌酶和Toll样受体mRNA的高表达水平直接或间接反映了日粮A. membranaceus或/和B. chinense对虾先天免疫的激活作用。然而， 在采用草药的治疗方法之间，在生长性能和免疫应答方面没有发现显着差异（P > 0.05）。总之，结果表明，A。membranaceus和B. chinense可用作白虾养殖的有益饲料添加剂和抗生素替代品。