Clostridium argentinense produces botulinum neurotoxin type G (BoNT/G). We sequenced and analyzed the plasmid harboring the bont/G gene, designated pCAG, in C. argentinense strain 2740. The pCAG consisted of 140,070 bp containing the bont/G gene cluster. Although this gene cluster showed high similarities in its DNA sequence and ORF arrangement to those of other bont gene clusters, the other regions of the plasmid did not. A phylogenetic study suggested that pCAG had a unique evolutionary history compared with other clostridial bont-harboring plasmids. This suggests that pCAG is possibly a novel type of plasmid expressing the bont/G gene in C. argentinense.

阿根廷梭菌产生 G 型肉毒杆菌神经毒素 (BoNT/G)。我们对阿根廷 C. 2740 菌株中含有bont/G基因的质粒进行了测序和分析，命名为 pCAG。pCAG 由 140,070 bp 组成，包含bont/G基因簇。尽管该基因簇在其 DNA 序列和 ORF 排列方面与其他bont基因簇表现出高度相似性，但质粒的其他区域却没有。系统发育研究表明，pCAG有一个独特的进化史与其他梭菌相比邦特-harboring质粒。这表明 pCAG 可能是一种在C. argentinense 中表达bont/G基因的新型质粒.