Introduction

Ketoacidosis of metabolic disease showed in beef cattle although body weight was increased by high-grain diets (HGDs). However, few studies have examined for health status related with metabolic disease of ketoacidosis following high-protein diet (HPD).

Objectives

Metabolomic analysis was performed for the monitoring of health status associated with metabolic disease of ketoacidosis in the plasma of Hanwoo heifers following a HPD.

Methods

Hanwoo heifers of 24 months with 459 ± 42 kg weight were used under a 2 × 2 crossover design. The plasma was collected from control (n = 5) and HPD group (n = 5) on day 21 following diet adaptation for 20 days. Metabolic profiling analysis of organic acids (OAs), amino acids (AAs) and fatty acids (FAs) by gas chromatography–tandem mass spectrometry combined with star pattern analysis was performed in plasma. Levels of OAs, AAs and FAs were evaluated by Mann-Whitney test, PCA and PLS-DA.

Results

In HPD group, ketoacidosis as metabolic disease was monitored by elevated acetoacetic acid and 3-hydroxybutyric acid. In addition, the elevation of ketogenic AAs, reduction of medium chain FAs and OAs with energy metabolism in TCA cycle were monitored in HPD group. Star graphic pattern was characteristic and readily distinguished between control and HPD groups. In PLS-DA, two groups were separated with VIP score of top-ranked 10 FAs as important metabolites for discrimination.

Conclusion

Elevation of ketone body including ketogenic AAs and reduced energy metabolism of FAs and OAs may useful for evaluation of health states associated with ketoacidosis from metabolic event by HPD in beef cattle.

中文翻译：

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高蛋白饮食下韩武牛肉血浆中有机酸、氨基酸和脂肪酸的代谢组学分析

介绍

尽管高粮日粮 (HGD) 增加了体重，但肉牛仍会出现代谢性疾病的酮症酸中毒。然而，很少有研究检查与高蛋白饮食（HPD）后酮酸中毒代谢疾病相关的健康状况。

目标

进行代谢组学分析以监测 HPD 后 Hanwoo 小母牛血浆中与酮症酸中毒代谢疾病相关的健康状况。

方法

在 2 × 2 交叉设计下使用 24 个月重 459 ± 42 kg 的 Hanwoo 小母牛。在饮食适应 20 天后的第 21 天，从对照组（n = 5）和 HPD 组（n = 5）收集血浆。通过气相色谱-串联质谱结合星型分析在血浆中对有机酸 (OA)、氨基酸 (AA) 和脂肪酸 (FA) 进行代谢谱分析。OAs、AAs 和 FAs 的水平通过 Mann-Whitney 检验、PCA 和 PLS-DA 进行评估。

结果

在 HPD 组中，酮症酸中毒作为代谢性疾病通过升高的乙酰乙酸和 3-羟基丁酸进行监测。此外，在HPD组中监测了生酮AA的升高，中链FA和OA在TCA循环中具有能量代谢的减少。星形图案具有特征性，易于区分对照组和 HPD 组。在 PLS-DA 中，以排名靠前的 10 种 FA 的 VIP 评分作为区分的重要代谢物，将两组分开。

结论

包括生酮 AA 在内的酮体升高以及 FA 和 OA 的能量代谢降低可能有助于评估肉牛 HPD 代谢事件引起的酮酸中毒相关的健康状况。