Purpose

To evaluate the effectiveness, efficiency, and safety of a transnational gamete donation (TGD) programme based on the shipping of vitrified donor oocytes.

Methods

A retro-prospective observational study was conducted in the Assisted Reproductive Technology Center of the University Hospital of Florence, Italy. The study population included 622 consecutive donor oocyte cycles. A mean number of 6 vitrified oocytes per couple were shipped from two Spanish biobanks. In the receiving centre, gametes were warmed and inseminated and the subsequent embryo transfer (ET) was performed. The main outcome measurement was LBR. Secondary outcomes included oocyte survival rate, ICSI damage rate, normal fertilization, cleavage, and implantation rate (IR) in both ‘fresh’ and cryotransfer cycles.

Results

A total number of 3536 mature oocytes were warmed with 81.4% surviving oocytes. 2PN oocytes were 1941 with an ICSI normal fertilization rate of 70.4% and a cleavage rate of 93.4%; 857 day-3 embryos were transferred in 498 women, 63 blastocysts in 44. Couples with at least one vitrified embryo were 181 (32.3%). IR was 25.1% and 33.1% for day-3 ET and blastocyst stage respectively. Crude pregnancy rate and LBR after the first ET were 35.5% and 27% correspondingly with a conservative cumulative LBR of 34% and an optimal LBR of 51.4%.

Conclusion

Imported vitrified donor oocytes retain their competence and are capable of resulting in ongoing pregnancies and healthy babies in a proportion comparable to other existing systems as egg donation with vitrification/warming in the same laboratory and transnational fresh oocyte donation.

中文翻译：

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“两个国家，两个实验室”：支持卵子捐赠的跨国配子捐赠（TGD）计划

目的

评估基于玻璃化供体卵母细胞运输的跨国配子捐赠（TGD）计划的有效性，效率和安全性。

方法

在意大利佛罗伦萨大学医院的辅助生殖技术中心进行了一项回顾性观察研究。研究人群包括622个连续的供体卵母细胞周期。每对夫妇平均从六个西班牙生物库中运出了6个玻璃化卵母细胞。在接收中心，将配子加热并授精，然后进行随后的胚胎移植（ET）。主要结局指标为LBR。次要结果包括“新鲜”和低温转移周期中的卵母细胞存活率，ICSI损伤率，正常受精，卵裂和着床率（IR）。

结果

总计3536个成熟卵母细胞与81.4％存活的卵母细胞一起被加热。2PN卵母细胞为1941年，ICSI正常受精率为70.4％，卵裂率为93.4％。在498名妇女中转移了857天的第3天胚胎，在44个中转移了63个胚泡。至少有一个玻璃化胚胎的夫妇有181个（32.3％）。第3天ET和胚泡期的IR分别为25.1％和33.1％。首次妊娠后的粗怀孕率和LBR分别为35.5％和27％，保守累积LBR为34％，最佳LBR为51.4％。

结论

进口的玻璃化供体卵母细胞保留了自己的能力，并能够以与其他现有系统相当的比例进行正在进行的怀孕和健康的婴儿，例如在同一实验室进行卵子化/保温和跨国新鲜卵母细胞捐赠。