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Comparison of the biocontrol efficacy of culture filtrate from Streptomyces philanthi RL-1-178 and acetic acid against Penicillium digitatum, in vitro and in vivo
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2020-10-12 , DOI: 10.1007/s10658-020-02128-0
Sawai Boukaew , Wanida Petlamul , Poonsuk Prasertsan

The antifungal compounds in a culture filtrate of Streptomyces philanthi RL-1-178 (culture filtrate RL-1-178) have been found to include acetic acid as their major component. The aim of this study was to compare the biocontrol efficacy of culture filtrate RL-1-178 with its major component at various concentrations, in inhibiting the growth of green mold disease from Penicillium digitatum, in vitro and in orange fruits. S. philanthi RL-1-178 was able to produce both antifungal metabolites and volatile compounds which inhibited the mycelial growth of P. digitatum. The effective dose of culture filtrate RL-1-178 was 2.5 mL/10 mL on PDA. Both culture filtrate RL-1-178 and commercial acetic acid at 1.5 mM completely suppressed the mycelial growth of P. digitatum and inhibited spore germination but did not kill the spores. Orange fruits treated with culture filtrate RL-1-178 showed less disease symptoms, disease incidence (12%) and a smaller lesion diameter (1.8 mm) than those treated with 1.5 mM acetic acid (25% and 5.6 mm, respectively). Moreover, a combination of both the culture filtrate and acetic acid (1.5 mM) was able to completely suppress the disease. The results revealed that culture filtrate RL-1-178 alone or acetic acid alone, as well as their combination were successful in controlling green mold disease.



中文翻译:

链霉菌RL-1-178培养液和乙酸在体外和体内对指状青霉的生物防治效果比较

已发现,在链霉菌RL-1-178的培养滤液(培养滤液RL-1-178)中的抗真菌化合物包括乙酸作为其主要成分。这项研究的目的是比较培养液RL-1-178及其主要成分在不同浓度下在抑制指状青霉在体外和在橙色水果中的绿色霉菌生长的生物防治功效。phil。philanthi RL-1-178能够产生抗真菌代谢产物和挥发性化合物,这些化合物可抑制指状疟原虫的菌丝体生长。在PDA上,培养滤液RL-1-178的有效剂量为2.5mL / 10mL。1.5 mM的培养滤液RL-1-178和市售乙酸均完全抑制了数位假单胞菌的菌丝生长并抑制了孢子萌发,但没有杀死孢子。与用1.5 mM乙酸处理(分别为25%和5.6 mm)处理的水果相比,用培养物滤液RL-1-178处理的橙色水果显示出较少的疾病症状,疾病发生率(12%)和较小的病灶直径(1.8 mm)。此外,培养滤液和乙酸(1.5 mM)的组合能够完全抑制该疾病。结果显示,单独的培养滤液RL-1-178或单独的乙酸,以及它们的组合成功地控制了绿霉病。

更新日期:2020-10-12
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