Abstract—DNA methylation has become an essential molecular approach to regulate gene expression through the regulation of methyl group addition/removal at the 5th position of Cytosine. The T1 progeny of T0 transgenic wheat plants were used to study the transgeneration methylation of promoter proximal regions of two exogenous promoters using bisulfite sequencing. The progeny of low and high T0 IFS expressers with high and low methylation levels respectively were used. Results of this study revealed that T1 plants that have 35S promoter driving the IFS inherited the methylation status of 35S promoter and IFS expression, especially methylation at the –56 and –88 CpG islands. On the other hand, the high IFS expresser of T0 plants with OL promoter driving the IFS expression passed the same pattern of IFS expression and methylation to their T1 progeny, whereas the low IFS expresser of T0 changed the pattern of expression and methylation to the high T0 expresser in their T1 progeny. This indicates that T1 wheat plants were able to demethylates DNA of the OL promoter proximal region, especially at –106 and –151 and reconstitute the IFS expression from low to high expresser through one generation. This also could indicate that plant promoters are more suitable for driving transgene in plant biotechnology. Results will improve our understanding of regulation of gene expression by DNA methylation and their application in plant biotechnology.

摘要-DNA甲基化已成为通过调控胞嘧啶第5位甲基的添加/去除来调控基因表达的重要分子方法。使用亚硫酸氢盐测序，使用T0转基因小麦植株的T1后代研究了两个外源启动子近端区域启动子的甲基化。使用分别具有高和低甲基化水平的低和高T0 IFS表达子代。这项研究的结果表明，具有驱动IFS的35S启动子的T1植物继承了35S启动子和IFS表达的甲基化状态，尤其是在–56和–88 CpG岛处的甲基化。另一方面，具有OL启动子驱动IFS表达的T0植物的高IFS表达体通过了相同的IFS表达模式和甲基化至其T1后代，而T0的低IFS表达子在其T1后代中将表达和甲基化的模式改变为高T0的表达子。这表明T1小麦植物能够使OL启动子近端区域的DNA脱甲基，尤其是在–106和–151处，并且可以通过一代将IFS的表达从低表达恢复为高表达。这也可能表明植物启动子更适合在植物生物技术中驱动转基因。结果将增进我们对通过DNA甲基化调节基因表达的理解及其在植物生物技术中的应用。特别是在–106和–151处，并通过一代重构了IFS从低表达到高表达的表达方式。这也可能表明植物启动子更适合在植物生物技术中驱动转基因。结果将增进我们对通过DNA甲基化调节基因表达的理解及其在植物生物技术中的应用。特别是在–106和–151处，并通过一代重构了IFS从低表达到高表达的表达方式。这也可能表明植物启动子更适合在植物生物技术中驱动转基因。结果将增进我们对通过DNA甲基化调节基因表达的理解及其在植物生物技术中的应用。