Abstract—Current experiment was conducted on existing cotton varieties against Cotton Leaf Curl Virus (CLCuV) and whitefly infection. The objective of this study was to determine the difference in leaf epicuticular wax load and composition of CLCuV resistant and susceptible cotton varieties. Settling behaviour of whitefly in previous studies showed more whitefly population on CLCuV susceptible whereas less on CLCuV resistant cotton plants. Correlation analysis of CLCuV infection with whitefly population and leaf epicuticular wax load was performed. CLCuV infection has significant positive correlation with whitefly population and significant negative correlation with leaf epicuticular wax load. These results depicted more leaf epicuticular wax load and less whitefly attack on G. arboreum as compared to G. hirsutum varieties. It was also hypothesized that resistant cotton varieties may have different wax composition than susceptible varieties. For this purpose, GC-MS analysis of leaf epicuticular wax of both types of varieties was performed. GC-MS results of CLCuV resistant and susceptible cotton plants also showed differences in quantity and composition of leaf epicuticular wax. Based on these results, we can conclude that leaf epicuticular wax acts as a barrier against CLCuV and whitefly infection. Different biochemical compounds present in leaf epicuticular wax along with their quantity, are also responsible for the resistance and susceptibility of G. arboreum and G. hirsutum varieties.

摘要—目前针对棉花品种的抗棉卷叶病毒（CLCuV）和粉虱感染进行了实验。这项研究的目的是确定抗CLCuV和易感棉花品种的叶片表皮蜡负荷和组成的差异。在以前的研究中，粉虱的沉降行为表明，在易受CLCuV侵害的棉株上，粉虱的种群较多，而抗CLCuV的棉株上的粉虱种群较少。进行了CLCuV感染与粉虱种群和叶片表皮蜡负荷的相关性分析。CLCuV感染与粉虱种群呈显着正相关，与叶片表皮蜡负荷呈显着负相关。这些结果表明，更多的叶片表皮蜡负荷和更少的粉虱侵染了G. arboreum与G. hirsutum品种相比。还假设抗性棉花品种的蜡组成可能与易感品种不同。为此，对两种类型的叶片表皮蜡进行了GC-MS分析。抗CLCuV和易感棉花植株的GC-MS结果也表明，叶表皮蜡的数量和组成存在差异。根据这些结果，我们可以得出结论，叶表皮蜡可作为对抗CLCuV和粉虱感染的屏障。叶片表皮蜡中存在的不同生化化合物及其数量，也负责植物阿波雷菌和陆地棉变种的抗性和敏感性。