The extraction of lipids from microalgae cells of Botryococcus braunii and Chlorella vulgaris after ultrasonic and microwave pretreatment was evaluated. Cell disruption increased the lipid extraction efficiency, and microwave pretreatment was more effective compared with ultrasonic pretreatment. The maximum lipid yield from B. braunii was 56.42% using microwave radiation and 39.61% for ultrasonication, while from C. vulgaris, it was respectively 41.31% and 35.28%. The fatty acid composition in the lipid extracts was also analyzed. The methane yield from the residual extracted biomass pretreated by microwaves ranged from 148 to 185 NmL CH₄/g VS for C. vulgaris and from 128 to 142 NmL CH₄/g VS for B. braunii. In the case of ultrasonic pretreatment, the methane production was between 168 and 208 NmL CH₄/g VS for C. vulgaris, while for B. braunii ranging from 150 to 174 NmL CH₄/g VS. Anaerobic digestion showed that lipid-extracted biomass presented lower methane yield than non-lipid-extracted feedstock, and higher amount of lipid obtained in the extraction contributed less methane production. Anyway, anaerobic digestion of the residual extracted biomass can be a suitable method to increase economic viability of energy recovery from microalgae.

进行了超声波和微波预处理后，评估了从Botryococcus braunii和Chlorella vulgaris微藻细胞中提取脂质的方法。细胞破裂增加了脂质提取效率，并且微波预处理比超声预处理更有效。利用微波辐射，来自芸苔芽孢杆菌的最大脂质产率为56.42％，用于超声处理的最大脂质产率为39.61％，而来自寻常型芽孢杆菌的最大脂质产率分别为41.31％和35.28％。还分析了脂质提取物中的脂肪酸组成。从通过微波预处理的残余提取的生物质的产率甲烷范围从148至185 NML CH ₄ /克VS为小球藻以及B. braunii的128至142 NmL CH ₄ / gVS。在超声预处理的情况下，甲烷产量为168和208之间NML CH ₄ /克VS为普通小球藻，而B.藻范围从150至174 NML CH ₄ /克VS. 厌氧消化表明，与非脂质提取的原料相比，脂质提取的生物量呈现出较低的甲烷收率，而提取中获得的脂质量越高，甲烷的产生量越少。无论如何，剩余提取生物质的厌氧消化可以是增加从微藻中回收能量的经济可行性的合适方法。