In order to obtain a new technology of on-site nitrate removal, a permeable reactive barrier (PRB) column was prepared by coupling the denitrifying bacteria with the use of agricultural waste. Nitrate removal in groundwater at low temperature (16 °C) and different hydraulic conditions was investigated in laboratory. Corncob was considered to be the most effective carbon source after the optimization experiments of nitrate removal. The PRB column was prepared by a 15-cm-high of corncob sandwiched between two layers of activated zeolite, where corncob was pre-mixed with denitrifying bacteria. The denitrification was studied at 16 °C and hydraulic retention time (HRT) ranged from 2.5 to 20.0 h, as well as the initial nitrate-N concentration in the range of 20–80 mg/L in groundwater. The values of NO₃⁻-N, NO₂⁻-N, NH₄⁺-N, and chemical oxygen demand (COD) and the optical density at a wavelength of 600 nm (OD₆₀₀) were measured as detection indexes. The results showed that the content of NO₃⁻-N and COD continued to decrease, but the NO₂⁻-N and NH₄⁺-N increased first and then decreased quickly in the effluent. The removal efficiency of NO₃⁻-N obtained 99.8%, 97.8%, 94.3%, and 85.9% at different initial nitrate-N of 20, 40, 60, and 80 mg/L and HTR of 24 h, respectively. The removal efficiency of NO₃⁻-N and COD was 83.2% and 95.7% at HRT of 20 h and initial nitrate-N of 70 mg/L. Therefore, the corncob PRB column combined comprehensively utilization of agricultural residues with remediation of nitrate, which was effective for nitrate removal in groundwater at site temperature and HRT.

为了获得一种现场去除硝酸盐的新技术，通过将反硝化细菌与农业废料结合使用，制备了可渗透反应性屏障（PRB）柱。在实验室中研究了低温（16°C）和不同水力条件下地下水中硝酸盐的去除。经过硝酸盐去除的优化实验后，玉米芯被认为是最有效的碳源。PRB色谱柱由夹在两层活性沸石之间的15厘米高的玉米芯制成，其中玉米芯与反硝化细菌预先混合。在16°C下研究了反硝化作用，水力停留时间（HRT）为2.5至20.0 h，以及地下水中硝酸盐-N的初始浓度为20-80 mg / L。NO ₃的值^-测定-N，NO ₂ ^-- N，NH ₄ ⁺ -N和化学需氧量（COD）以及在600nm的波长下的光密度（OD ₆₀₀）作为检测指标。结果表明，污水中NO ₃ ^-- N和COD的含量持续下降，但出水NO ₂ ^-- N和NH ₄ ⁺ -N先增加后迅速减少。在不同的初始硝酸盐-N为20、40、60和80 mg / L和HTR为24 h时，NO ₃ ^-- N的去除效率分别为99.8％，97.8％，94.3％和85.9％。NO的去除率₃ ^-在20 h的HRT和70 mg / L的初始硝态氮下，-N和COD分别为83.2％和95.7％。因此，玉米芯PRB柱将农业残留物的综合利用与硝酸盐的修复相结合，在现场温度和HRT的条件下有效去除了地下水中的硝酸盐。