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Enhancement of Galactose Uptake from Kappaphycus alvarezii Using Saccharomyces cerevisiae through Deletion of Negative Regulators of GAL Genes
Applied Biochemistry and Biotechnology ( IF 3 ) Pub Date : 2020-10-12 , DOI: 10.1007/s12010-020-03434-3
In Yung Sunwoo , Pailin Sukwong , Yu Rim Park , Deok Yeol Jeong , Soo Rin Kim , Gwi-Teak Jeong , Sung-Koo Kim

This study was aimed at enhancing galactose consumption from the red seaweed Kappaphycus alvarezii. The optimal pretreatment condition of thermal acid hydrolysis was treated with 350 mM HNO3 for 60 min at 121 °C. The enzymatic saccharification with a 1:1 mixture of Celluclast 1.5 L and Viscozyme L showed the maximum yield of glucose; 42-g/L monosaccharide concentration was obtained with the highest yield of pretreatment and enzymatic saccharification (EPS) and the lowest inhibitory compound concentration. The deletion of the GAL80, MIG1, CYC8, or TUP1 gene was performed to improve the galactose consumption rate. The strains with the deletion of the MIG1 gene (mig1Δ) showed higher galactose consumption rate and ethanol yield than other strains. High transcription levels of regulatory genes revealed that the mig1Δ relieved glucose repression. These results show that the mig1Δ enhances galactose consumption rate from K. alvarezii.



中文翻译:

酿酒酵母通过删除GAL基因的负调控子,提高了从Kappaphycus alvarezii吸收半乳糖的能力

这项研究旨在提高红海藻Kappaphycus alvarezii的半乳糖消耗量热酸水解的最佳预处理条件是在121°C下用350 mM HNO 3处理60分钟。用Celluclast 1.5 L和Viscozyme L的1:1混合物进行酶促糖化显示了最大的葡萄糖产量。以最高的预处理和酶促糖化(E PS)收率和最低的抑制化合物浓度获得了42 g / L的单糖浓度。进行GAL80MIG1CYC8TUP1基因的缺失以提高半乳糖消耗率。带有缺失的菌株MIG1基因(mig1Δ)显示出比其他菌株更高的半乳糖消耗率和乙醇产量。调节基因的高转录水平表明mig1Δ减轻了葡萄糖的阻遏。这些结果表明,mig1Δ提高了K. alvarezii的半乳糖消耗率。

更新日期:2020-10-12
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