In this study, the aerobic activated sludge for skatole removal was enriched from pig slurry in three parallel sequencing batch reactors. The sludge system exhibited a satisfactory performance for skatole removal during the 40 days operation. High-throughput sequencing results showed that the α-diversity remained unchanged before and after the operation process. However, the structures of bacterial and fungal communities notably shifted. Particularly, Arthrobacter increased to be the major bacterial genus from 2.15 ± 0.76% (day 0) to 23.80 ± 24.36% (day 40), and Fusicolla became the major fungal genus from 1.20 ± 0.48% (day 0) to 37.17 ± 7.47% (day 40). These results indicated that Arthrobacter and Fusicolla might participate in skatole removal in sludge systems, though both genera were not reported to be able to degrade skatole. This is the first study describing skatole-degrading bacterial and fungal communities in the enrichment from pig slurry to the best of our knowledge, providing important guidance for skatole control and bioremediation.

在这项研究中，用于去除粪臭素的好氧活性污泥在三个平行的序批式反应器中从猪粪中富集。在 40 天的运行过程中，污泥系统表现出令人满意的去除粪臭素的性能。高通量测序结果显示，α-多样性在手术前后均保持不变。然而，细菌和真菌群落的结构发生了显着变化。特别是，节杆菌从 2.15±0.76%（第 0 天）增加到 23.80±24.36%（第 40 天），成为主要真菌属，梭杆菌从 1.20±0.48%（第 0 天）增加到 37.17%±7 （第 40 天）。这些结果表明节杆菌属和梭菌属可能参与污泥系统中的粪臭素去除，尽管据报道这两个属都不能降解粪臭素。据我们所知，这是第一项描述从猪粪中富集粪臭素降解细菌和真菌群落的研究，为粪臭素控制和生物修复提供了重要指导。