The effect of partial hybridization and probe positioning on the performance of a label free biosensor has been investigated in this article. Dielectric modulated dual-metal double-gate oxide stacked junctionless MOSFET based biosensor device has been considered as a reference during analysis. Primarily, an optimized device dimension has been proposed based on device parameters, cavity positions, etc. Later, Sensitivity parameter variations in terms of threshold voltage (VTH) and drain current (IDS) have been investigated by considering partial hybridization (PH) and arrangement of receptor-biotarget pairs. Analytical modeling has been detailed to study the performance of the sensor. Extensive simulations using the SILVACO ATLAS investigate the effects. Mainly, irregular arrangements of biomolecules like an enzyme (Staphylococcal nuclease) and DNA (negatively-charged) are examined here. Also, the minimum detection limit of the biosensor has been addressed including a capacitive model. It was observed that the optimized cavity with dimension (400 nm × 10 nm) can accommodate almost 1.016 × 105 (∼ 17 aM) number of Myoglobin enzyme. Analysis of these practical issues during the label free detection of charged/neutral biomolecules with higher sensitivity would be efficacious to find out the promising alternative in biosensing applications.

中文翻译：

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介电调制无结无标记生物传感器灵敏度的部分杂交和探针定位分析

本文研究了部分杂交和探针定位对无标记生物传感器性能的影响。介电调制的双金属双栅极氧化物堆叠无结 MOSFET 生物传感器装置已被视为分析过程中的参考。首先，基于器件参数、腔位置等提出了优化的器件尺寸。后来，通过考虑部分杂化 (PH) 和排列，研究了阈值电压 (VTH) 和漏电流 (IDS) 方面的灵敏度参数变化受体-生物靶标对。已详细分析建模以研究传感器的性能。使用 SILVACO ATLAS 的广泛模拟研究了这些影响。主要是，这里检查了生物分子的不规则排列，如酶（葡萄球菌核酸酶）和 DNA（带负电荷）。此外，还解决了生物传感器的最低检测极限，包括电容模型。据观察，尺寸（400 nm × 10 nm）的优化腔可以容纳几乎 1.016 × 105 (∼ 17 aM) 数量的肌红蛋白酶。在具有更高灵敏度的带电/中性生物分子的无标记检测过程中分析这些实际问题将有效地找到生物传感应用中的有希望的替代方案。肌红蛋白酶的数量为 016 × 105 (∼ 17 aM)。在具有更高灵敏度的带电/中性生物分子的无标记检测过程中分析这些实际问题将有效地找到生物传感应用中的有希望的替代方案。肌红蛋白酶的数量为 016 × 105 (∼ 17 aM)。在具有更高灵敏度的带电/中性生物分子的无标记检测过程中分析这些实际问题将有效地找到生物传感应用中的有希望的替代方案。