Zn²⁺, as the second most abundant d-block metal in the human body, plays an important role in a wide range of biological processes, and the dysfunction of its homeostasis is related to many diseases, including Type 2 diabetes, Alzheimer's disease and prostate and breast cancers. Small molecule fluorescent probes, as effective tools for real-time imaging, have been widely used to study Zn²⁺ related processes. However, the failure to control their localisation in cells has limited their utility somewhat, as they are generally incapable of studying individual processes in a specific cellular location. This perspective presents an overview of the recent developments in specific organelle localised small molecule fluorescent Zn²⁺ probes and their application in biological milieu, which could help to extend our understanding of the mechanisms that cells use to respond to dysfunction of zinc homeostasis and its roles in disease initiation and development.

中文翻译：

---

亚细胞定位小分子荧光探针对移动 Zn2+ 进行成像

Zn ²⁺作为人体内第二丰富的 d 区金属，在多种生物过程中发挥着重要作用，其稳态功能障碍与许多疾病有关，包括 2 型糖尿病、阿尔茨海默病和前列腺癌和乳腺癌。小分子荧光探针作为实时成像的有效工具，已被广泛用于研究Zn ²⁺相关过程。然而，无法控制它们在细胞中的定位在一定程度上限制了它们的实用性，因为它们通常无法研究特定细胞位置中的单个过程。该观点概述了特定细胞器局部小分子荧光 Zn ²⁺探针的最新进展及其在生物环境中的应用，这可能有助于扩展我们对细胞响应锌稳态功能障碍及其作用的机制的理解在疾病的发生和发展中。