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Visible-light triggered templated ligation on surface using furan-modified PNAs
Chemical Science ( IF 8.4 ) Pub Date : 2020-10-09 , DOI: 10.1039/d0sc04875e
Alex Manicardi 1 , Enrico Cadoni 1 , Annemieke Madder 1
Affiliation  

Oligonucleotide-templated reactions are frequently exploited for target detection in biosensors and for the construction of DNA-based materials and probes in nanotechnology. However, the translation of the specifically used template chemistry from solution to surfaces, with the final aim of achieving highly selective high-throughput systems, has been difficult to reach and therefore, poorly explored. Here, we show the first example of a visible light-triggered templated ligation on a surface, employing furan-modified peptide nucleic acids (PNAs). Tailored photo-oxidation of the pro-reactive furan moiety is ensured by the simultaneous introduction of a weak photosensitizer as well as a nucleophilic moiety in the reacting PNA strand. This allows one to ensure a localized production of singlet oxygen for furan activation, which is not affected by probe dilution or reducing conditions. Simple white light irradiation in combination with target-induced proximity between reactive functionalities upon recognition of a short 22mer DNA or RNA sequence that functions as a template, allows sensitive detection of nucleic acid targets in a 96 well plate format.

中文翻译:

使用呋喃修饰的 PNA 进行可见光触发表面模板化连接

寡核苷酸模板反应经常用于生物传感器中的目标检测以及纳米技术中基于 DNA 的材料和探针的构建。然而,将专门使用的模板化学从溶液到表面的转化,以实现高度选择性的高通量系统的最终目标,一直难以实现,因此,探索很少。在这里,我们展示了使用呋喃修饰的肽核酸(PNA)在表面上进行可见光触发模板化连接的第一个例子。通过在反应性 PNA 链中同时引入弱光敏剂和亲核部分,确保了前反应性呋喃部分的定制光氧化。这使得人们能够确保用于呋喃活化的单线态氧的局部产生,而不受探针稀释或还原条件的影响。简单的白光照射与识别作为模板的短 22mer DNA 或 RNA 序列后靶标诱导的反应功能之间的接近相结合,可以在 96 孔板格式中灵敏地检测核酸靶标。
更新日期:2020-10-11
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