There are few existing methods for shortening the decellularization period for a human-sized whole-liver scaffold. Here, we describe a protocol that enables effective decellularization of the liver obtained from pigs weigh 120 ± 4.2 kg within 72 h. Porcine livers (approx. 1.5 kg) were decellularized for 3 days using a combination of chemical and enzymatic decellularization agents. After trypsin, sodium deoxycholate, and Triton X-100 perfusion, the porcine livers were completely translucent. Our protocol was efficient to promote cell removal, the preservation of extracellular matrix (ECM) components, and vascular tree integrity. In conclusion, our protocol is efficient to promote human-sized whole-liver scaffold decellularization and thus useful to generate bioengineered livers to overcome the shortage of organs.

中文翻译：

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优化去细胞猪肝支架方案

现有的用于缩短人体全肝支架去细胞化时间的方法很少。在这里，我们描述了一个协议，该协议能够在 72 小时内对重 120 ± 4.2 公斤的猪的肝脏进行有效的去细胞化。使用化学和酶促脱细胞剂的组合将猪肝脏（约 1.5 kg）脱细胞 3 天。在胰蛋白酶、脱氧胆酸钠和 Triton X-100 灌注后，猪肝脏完全透明。我们的协议有效地促进了细胞去除、细胞外基质 (ECM) 成分的保存和血管树的完整性。总之，我们的方案有效地促进了人体大小的全肝支架去细胞化，因此有助于生成生物工程肝脏以克服器官短缺。