The etiological agent of human amoebiasis is the protozoan parasite E. histolytica; the disease is still an endemic infection in some countries and the outcome of infection in the host infection can range from asymptomatic intestinal infection to intestinal or liver invasive forms of the disease. The invasive character of this parasite is multifactorial and mainly due to the differential expression of multiple pathogenic genes. The aim of the present work was to measure the differential expression of some genes in different specimens of patients with amoebic liver abscess (ALA) and specimens of genital amoebiasis (AG) by RT-qPCR. Results show that the expression of genes is different in both types of samples. Almost all studied genes were over expressed in both sets of patients; however, superoxide dismutase (Ehsod), serine threonine isoleucine rich protein (Ehstirp), peroxiredoxin (Ehprd) and heat shock protein 70 and 90 (Ehhsp-70, EHhsp-90) were higher in AG biopsies tissue. Furthermore, cysteine proteinases 5 and 2 (Ehcp5, Ehcp2), lectin (Ehgal/galnaclectin) and calreticulin (Ehcrt) genes directly associate with pathogenic mechanisms of E. histolytica had similar over expression in both AG and ALA samples. In summary the results obtained show that trophozoites can regulate the expression of their genes depending on stimuli or environmental conditions, in order to regulate their pathogenicity and ensure their survival in the host.

中文翻译：

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不同临床形式阿米巴病患者溶血性大肠杆菌的致病基因差异表达

人类阿米巴病的病因是原生动物寄生虫溶血性大肠杆菌。在某些国家，该疾病仍然是地方性感染，宿主感染的结果可能从无症状的肠道感染到肠道或肝脏侵袭性疾病形式不等。该寄生虫的侵袭特性是多因素的，并且主要归因于多种致病基因的差异表达。本工作的目的是通过RT-qPCR测定不同基因在阿米巴肝脓肿患者（ALA）和生殖器阿米巴病（AG）患者中某些基因的差异表达。结果表明，两种样品中基因的表达均不同。两组患者中几乎所有研究的基因均过表达；但是，超氧化物歧化酶（Ehsod），富含丝氨酸苏氨酸异亮氨酸的蛋白质（Ehstirp），过氧化物酶（Ehprd）以及热休克蛋白70和90（Ehhsp-70，EHhsp-90）在AG活检组织中较高。此外，半胱氨酸蛋白酶5和2（Ehcp 5，Ehcp 2），凝集素（Ehgal / galnaclectin）和钙网蛋白（Ehcrt）基因直接与溶组织性大肠杆菌的致病机制相关。在AG和ALA样品中都有相似的过表达。总而言之，获得的结果表明，滋养体可以根据刺激或环境条件调节其基因的表达，从而调节其致病性并确保其在宿主中的存活。