Abstract

Three Apulian (SE Italy) coastal sites, Torre Canne to Torre San Leonardo (T), Punta della Contessa Salt Pans (S) and the La Vela Swamp (P), characterised by sandy shores and salt marshes recognised as wetland environments belonging to the Natura 2000 network, were selected as study areas in order to survey the vegetation zonation, plant communities, ecological context and genetic diversity of Thinopyrum elongatum population within humid habitats. Analysis of vegetation, plant communities and soil samplings were carried out to investigate the ecological context of 24 populations collected of this halophile wheatgrass, along with biochemical characterization (A-PAGE, SDS-PAGE, and IEFxSDS-PAGE) of seed storage proteins, used to assess the genetic diversity intra- and inter-populations and genotype frequencies. Thinopyrum elongatum populations showed a significative presence in the halophile shrub plant communities of salt marshes, especially those characterised by Limbarda crithmoides and Artemisia caerulescens. The differences of the protein patterns, suggest the presence in the T area of a higher genetic variability and a greater resistance to the numerous factors of human pressure. The results have been discussed hypothesizing that this multidisciplinary approach could be considered a model to evaluate in the future the same environments or other similar ones, subjected to anthropogenic pressures and environmental changes.

摘要

三个普利亚（意大利东南部）沿海站点，Torre Canne 到 Torre San Leonardo (T)、Punta della Contessa Salt Pans (S) 和 La Vela Swamp (P)，其特点是沙质海岸和盐沼，被认为是属于选择Natura 2000网络作为研究区域，以调查湿润生境内长穗刺麦草种群的植被分区、植物群落、生态环境和遗传多样性。对植被、植物群落和土壤采样进行了分析，以调查从这种嗜盐小麦草中采集的 24 个种群的生态环境，以及种子储存蛋白的生化特征（A-PAGE、SDS-PAGE 和 IEFxSDS-PAGE），用于评估种群内和种群间的遗传多样性以及基因型频率。在盐沼的嗜盐灌木植物群落中，特别是那些以 Limbarda crithmoides 和 Artemisia caerulescens 为特征的植物群落中，长穗刺槐种群表现出显着的存在。蛋白质模式的差异表明 T 区域存在更高的遗传变异性和对人类压力的众多因素的更大抵抗力。已经讨论了结果，假设这种多学科方法可以被视为未来评估相同环境或其他类似环境的模型，这些环境会受到人为压力和环境变化的影响。