Abstract

Aminoglycoside antibiotics have been used for treating serious but also routine infections in veterinary and human medicine for many years. The basic aim of this work is to evaluate the cytotoxicity of dihydrostreptomycin and neomycin in vitro on three cell cultures - BHK-21 (Syrian golden hamster kidney fibroblast), VERO (African green monkey kidney fibroblast) and FEA (feline embryonic fibroblast) cells. The morphological changes were examined by Giemsa staining. Cells were dried and visualized under fluorescence microscope. After the exposure to different experimental doses of dihydrostreptomycin (812.5-20000 µg/mL) and neomycin (1000-20000 µg/mL) during 24 h, the viability of BHK-21, FEA and VERO cell lines were evaluated by MTT assay. Viability of BHK-21 cells significantly (P < 0.001) decreased after treatment with 3500; 5500 and 7500 µg/mL of dihydrostreptomycin and 9000; 10000 and 20000 µg/mL of neomycin. The FEA cell viability decreased significantly (P < 0.001; P < 0.01) at 2500 and 3000 µg/mL dihydrostreptomycin and at 3000 µg/mL of neomycin treatment. Only the highest concentration of dihydrostreptomycin (20000 µg/mL) reduced VERO cell viability significantly (P < 0.01). Based on or results we can assume the effect of different antibiotics in different concentrations on cell lines is various. Detection of antibiotic toxicity to animal cells is very important because of the increasing resistance of bacteria. One of the solutions is drug dose increasing, but only to a certain concentration, since the toxic effect over the therapeutic one will prevail, which we have also shown in this work.

摘要

多年来，氨基糖苷类抗生素已被用于治疗兽用和人用药物中的严重感染和常规感染。这项工作的基本目的是评估二氢链霉素和新霉素的体外细胞毒性。在三种细胞培养物中-BHK-21（叙利亚金仓鼠肾成纤维细胞），VERO（非洲绿猴肾成纤维细胞）和FEA（猫胚胎成纤维细胞）细胞。通过吉姆萨染色检查形态变化。将细胞干燥并在荧光显微镜下观察。在24小时内暴露于不同实验剂量的二氢链霉素（812.5-20000μg/ mL）和新霉素（1000-20000μg/ mL）之后，通过MTT分析评估BHK-21，FEA和VERO细胞系的生存力。3500处理后，BHK-21细胞的活力显着下降（P <0.001）；5500和7500 µg / mL的二氢链霉素和9000；10000和20000 µg / mL新霉素。在2500和3000 µg / mL二氢链霉素以及在3000 µg / mL新霉素处理下，FEA细胞活力显着降低（P <0.001； P <0.01）。只有最高浓度的二氢链霉素（20000 µg / mL）才能显着降低VERO细胞活力（P <0.01）。基于或得出的结果，我们可以假设不同浓度的不同抗生素对细胞系的影响是不同的。由于细菌抵抗力的增强，检测抗生素对动物细胞的毒性非常重要。解决方案之一是增加药物剂量，但只能增加到一定浓度，因为对治疗药物的毒性作用将占上风，我们在这项工作中也已证明了这一点。由于细菌抵抗力的增强，检测抗生素对动物细胞的毒性非常重要。解决方案之一是增加药物剂量，但只能增加到一定浓度，因为对治疗药物的毒性作用将占上风，我们在这项工作中也已证明了这一点。由于细菌抵抗力的增强，检测抗生素对动物细胞的毒性非常重要。解决方案之一是增加药物剂量，但只能增加到一定浓度，因为对治疗药物的毒性作用将占上风，我们在这项工作中也已证明了这一点。