Abstract

Objective

The cerebral ischemia-reperfusion (I/R) model is crucial for the study of cerebral stroke. Chrysophanol (Chry) can protect nerve damage of mice in cerebral ischemia-reperfusion injury. This study aimed at investigating the neuroprotective effects of chrysophanol through mitochondrial autophagy in mice with ischemia-reperfusion injury.

Materials and methods

Adult mice were stochastically divided into five groups: sham, I/R (solvent), I/R+Chry (dose, 10.0ml/kg), I/R+Chry (dose, 1.0ml/kg), and I/R+Chry (dose, 0.1ml/kg). The cerebral ischemia-reperfusion model was made in I/R and I/R+Chry groups. The changes in hippocampal formation were observed by hematoxylin and eosin (H&E) staining. The expressions of LC3B-II and LC3B-I protein in hippocampus were demonstrated by western blot (WB). The fluorescence intensities of NIX, LC3B, and mitochondria were detected by immunohistochemistry fluorescent (IF).

Results

Comparing with the I/R group, the I/R+Chry groups showed improvements in reducing the damage on the hippocampus, indicated by the reduced ratio of LC3B-II and LC3B-I protein, decreased fluorescence intensity of NIX and LC3B, and increased intensity of mitochondrial fluorescence.

Conclusion

Our study showed that chrysophanol may regulate mitochondrial autophagy through NIX protein and alleviate the damage of hippocampus through decreasing the level of mitochondrial autophagy.

中文翻译：

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大黄酚对脑缺血再灌注小鼠海马损伤及线粒体自噬的影响

摘要

客观的

脑缺血再灌注 (I/R) 模型对于脑卒中的研究至关重要。大黄酚(Chry)对脑缺血再灌注损伤小鼠的神经损伤有保护作用。本研究旨在研究大黄酚通过线粒体自噬对缺血再灌注损伤小鼠的神经保护作用。

材料和方法

成年小鼠随机分为五组：sham、I/R（溶剂）、I/R+Chry（剂量，10.0ml/kg）、I/R+Chry（剂量，1.0ml/kg）和I/R +Chry（剂量，0.1ml/kg）。I/R组和I/R+Chry组制作脑缺血再灌注模型。通过苏木精和伊红（H＆E）染色观察海马形成的变化。免疫印迹（WB）证实了海马LC3B-II和LC3B-I蛋白的表达。NIX、LC3B和线粒体的荧光强度通过免疫组织化学荧光（IF）检测。

结果

与 I/R 组相比，I/R+Chry 组在减少海马损伤方面表现出改善，表现为 LC3B-II 和 LC3B-I 蛋白的比例降低，NIX 和 LC3B 的荧光强度降低，并增加线粒体荧光强度。

结论

我们的研究表明，大黄酚可能通过NIX蛋白调节线粒体自噬，通过降低线粒体自噬水平来减轻海马损伤。