Abstract

Alcohol and its metabolites are responsible for damage both within the gastrointestinal tract and other organs. Alcohol abuse promote intestinal inflammation, that may be the cause of multiple organ dysfunctions and chronic disorders. In this research, the effect of astaxanthin, a powerful antioxidant with several biological effects, on alcohol damage-induced in the intestine of Carassius auratus, was investigated. In the fishes exposed to ethanol, an increase of the intestinal epithelium mucous cells and circulating macrophages, with intestinal mucosa disorganization was observed. In contrast, in the fishes treated with astaxanthin intestinal morphology was restored. By immunohistochemical analysis, using α-Smooth Muscle Actin (α-SMA) and Vasoactive intestinal polypeptide (VIP) antibodies, a reduction of inflammatory states alcohol-induced was evident, with more regular muscularis submucosa and more organized intestinal mucosa without inflammatory cells. The results suggest that astaxanthin treatments can be a good candidate for preventing damage within the gastrointestinal associated with excessive alcohol consumption.

摘要

酒精及其代谢物对胃肠道和其他器官造成损害。酗酒会促进肠道炎症，这可能是导致多器官功能障碍和慢性疾病的原因。在这项研究中，虾青素是一种具有多种生物效应的强抗氧化剂，对鲫鱼肠道酒精损伤的影响，进行了调查。在暴露于乙醇的鱼中，观察到肠上皮黏液细胞和循环巨噬细胞增加，肠黏膜紊乱。相比之下，用虾青素处理的鱼的肠道形态得到了恢复。通过免疫组织化学分析，使用 α-平滑肌肌动蛋白 (α-SMA) 和血管活性肠多肽 (VIP) 抗体，酒精诱导的炎症状态明显减少，粘膜下层肌层更规则，肠粘膜组织更有序，没有炎症细胞。结果表明，虾青素治疗可以很好地预防与过量饮酒相关的胃肠道损伤。