Abstract

Annona reticulata is native to South and Central America which has many phytochemical and pharmacological activities suggesting a wide range of clinical application in lieu of cancer chemotherapy. This study provides abundant genomic data for the genetic relationship study, germplasm resources evaluation and varieties selection of A. reticulata. The complete chloroplast genome of A. reticulata was sequenced, assembled, and annotated in this study. The genome size was 201,906 bp and was divided into four regions: a large single-copy region of 69,650 bp, a small single-copy region of 3,014 bp, and two inverted repeat regions of 64,621 bp. A total number of 164 genes were annotated, including 115 protein-coding genes, one pseudogene, 40 tRNA genes, and eight rRNA genes. In terms of gene function, the 164 genes were divided into four major groups: genes for self-replication, photosynthesis, unknown function, and other genes. A maximum likelihood tree based on the chloroplast genome sequences of 24 plant species was constructed. The result of phylogenetic analysis showed that A. cherimola had the closest relationship with A. reticulate.

摘要

网状Annona reticulata原产于南美和中美洲，具有许多植物化学和药理活性，表明可广泛用于代替癌症化学疗法的临床应用。本研究为网纹拟南芥的遗传关系研究，种质资源评价和品种选择提供了丰富的基因组数据。网纹拟南芥完整的叶绿体基因组在这项研究中被测序，组装和注释。基因组大小为201,906 bp，分为四个区域：一个较大的单拷贝区域，为69,650 bp，一个较小的单拷贝区域，为3,014 bp，以及两个反向重复区域，分别为64,621 bp。共注释了164个基因，包括115个蛋白质编码基因，一个假基因，40个tRNA基因和8个rRNA基因。在基因功能方面，这164个基因分为四个主要类别：自我复制基因，光合作用基因，未知功能基因和其他基因。基于24种植物叶绿体基因组序列构建了最大似然树。系统发育分析结果表明，圆曲霉与网状曲霉关系最为密切。