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Atg8-mediated super-assembly of Atg40 induces local ER remodeling in reticulophagy
Autophagy ( IF 13.3 ) Pub Date : 2020-10-10 , DOI: 10.1080/15548627.2020.1831801
Keisuke Mochida 1 , Hitoshi Nakatogawa 1
Affiliation  

ABSTRACT

Reticulophagy (or ER-phagy) is a type of selective autophagy that targets the endoplasmic reticulum (ER). In the process of reticulophagy, part of the ER is fragmented and packed within autophagosomes. However, the underlying mechanism that induces this local remodeling of ER subdomains was poorly understood. Our recent study showed that in the budding yeast Saccharomyces cerevisiae the reticulophagy receptor Atg40 plays an important role in ER remodeling beyond its role as a tether between the ER and the phagophore [1 MochidaK, YamasakiA, MatobaK, et al. Super-assembly of ER-phagy receptor Atg40 induces local ER remodeling at contacts with forming autophagosomal membranes. Nat Commun. 2020;11:3306.[Crossref], [PubMed], [Web of Science ®] , [Google Scholar]]. Atg40 has an ability to generate positive membrane curvature through the reticulon-like domain and locally forms a super assemblage though its binding to Atg8 at ER-phagophore contacts. These Atg40 assemblages cause folding of the ER subdomains to allow them to be efficiently packed into autophagosomes. Furthermore, our structural analysis identified an evolutionarily conserved short helix that assists strong Atg8-binding of reticulophagy receptors.



中文翻译:

Atg8 介导的 Atg40 超组装诱导网状吞噬中的局部 ER 重塑

摘要

网状自噬(或内质网自噬)是一种靶向内质网 (ER) 的选择性自噬。在网状吞噬过程中,部分内质网被打断并堆积在自噬体内。然而,诱导 ER 子域局部重塑的潜在机制知之甚少。我们最近的研究表明,在出芽酵母酿酒酵母中,网状吞噬受体 Atg40 在 ER 重塑中发挥着重要作用,超出了其作为 ER 和吞噬细胞之间的系绳的作用 [ 1] Mochida KYamasaki AMatoba K等。内质网自噬受体 Atg40 的超组装在与形成自噬体膜的接触处诱导局部内质网重塑。纳特社区。2020 年;11:3306[Crossref]、[PubMed]、[Web of Science®]、 [Google Scholar]]。Atg40 具有通过网状结构域产生正膜曲率的能力,并通过其在 ER-吞噬细胞接触处与 Atg8 结合而局部形成超级组合。这些 Atg40 组合导致 ER 子域折叠,使它们能够有效地装入自噬体。此外,我们的结构分析确定了一个进化上保守的短螺旋,它有助于网状吞噬受体的强 Atg8 结合。

更新日期:2020-12-17
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