In this work, we reported a simple coumarin‐boronic acid ester fluorescent probe 1, which displayed very high “ON‐OFF” fluorescence response for Hg²⁺ in an aqueous environment (H₂O/CH₃CN = 1:1, v/v) or in a PBS solution (pH = 7.4). With the addition of Hg²⁺ to the solution of probe, a color change from light yellow to orange could be observed by naked eye. The detection limits were lower than the permitted level of Hg²⁺ in drinking water defined by WHO. Infrared spectroscopy, mass spectroscopy and ¹H NMR titration studies showed that probe 1 formed a 1:1 complex with Hg²⁺. In addition, the probe was non‐toxic to cells and could stain specifically the membrane region surrounding the HeLa cell.

中文翻译：

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基于香豆素-硼的荧光“ ON-OFF”探针用于水溶液中的Hg2 +

在这项工作中，我们报道了一个简单的香豆素-硼酸酯荧光探针1，在水环境中，Hg ²⁺表现出非常高的“ ON-OFF”荧光响应（H ₂ O / CH ₃ CN = 1：1，v / v）或在PBS溶液中（pH = 7.4）。将Hg ^2+添加到探针溶液中，用肉眼可以观察到从浅黄色到橙色的颜色变化。检出限低于世界卫生组织规定的饮用水中Hg ²⁺的允许水平。红外光谱，质谱和¹ H NMR滴定研究表明，探针1与Hg ²⁺形成1：1的络合物。此外，该探针对细胞无毒，可以特异性染色HeLa细胞周围的膜区域。